Key features and details
- Rabbit polyclonal to TRAM1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TRAM1 antibody
See all TRAM1/TRAM primary antibodies
DescriptionRabbit polyclonal to TRAM1
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
- WB: H1299 whole cell lysate. IHC: mouse intestinal and AGS xenograft tissue. ICC/IF: HeLa cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 43 kDa.|
RelevanceFunction: Stimulatory or required for the translocation of secretory proteins across the ER membrane. Similarity: Belongs to the TRAM family. Contains 1 TLC (TRAM/LAG1/CLN8) domain.
Cellular localizationEndoplasmic reticulum membrane; Multipass membrane protein
- PNAS 8 antibody
- PNAS8 antibody
- PRO1292 antibody
Anti-TRAM1 antibody (ab96106) at 1/1000 dilution + H1299 cell lysate at 30 µg
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse intestine staining TRAM1 with ab96106 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence of methanol-fixed HeLa cells staining TRAM1 with ab96106 at 1/500 dilution (green). Costained with Hoechst 33342 (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of AGS xenograft staining TRAM1 with ab96106 at 1/500 dilution.
ab96106 has been referenced in 3 publications.
- Wu X et al. Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination. Nat Commun 10:4115 (2019). PubMed: 31511519
- Iampietro M et al. Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection. PLoS Pathog 13:e1006397 (2017). PubMed: 28542576
- Yew KH et al. Human cytomegalovirus induces TLR4 signaling components in monocytes altering TIRAP, TRAM and downstream interferon-beta and TNF-alpha expression. PLoS One 7:e44500 (2012). WB . PubMed: 22970235