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Rabbit polyclonal to Transcription factor 25
HumanPredicted to work with:
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 427 and 648 of Transcription factor 25 (NP_055787)
- Hela whole cell lysate, cultured HeLa cells, FaDu xenograft. 293T and Molt-4 cell lines.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 1.21% Tris, 0.75% Glycine
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 77 kDa.
||1/100 - 1/250.
||1/100 - 1/200.
May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development.
In the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart.
Belongs to the TCF25 family.
The C-terminal region mediates transcriptional repression.
Nucleus. Some staining in the cytosol.
Information by UniProt
- FKSG26 antibody
- hKIAA1049 antibody
- Hulp1 antibody
Western blot - Anti-Transcription factor 25 antibody (ab96813)
Anti-Transcription factor 25 antibody (ab96813) at 1/500 dilution + HeLa whole cell lysate at 30 µgPredicted band size:
7.5% SDS Page
Secondary antibody - anti-rabbit HRP (ab6721)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor 25 antibody (ab96813)
Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using ab96813 antibody at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Transcription factor 25 antibody (ab96813)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96813 antibody at 1/200 dilution. Bottom picture is the same as the top one but is merged with fluorescent DNA probe.
has not yet been referenced specifically in any publications.
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