Key features and details
- Rabbit polyclonal to Transcription factor AP-2-alpha
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Transcription factor AP-2-alpha antibody
See all Transcription factor AP-2-alpha primary antibodies
DescriptionRabbit polyclonal to Transcription factor AP-2-alpha
Specificityab52222 detects endogenous levels of total Transcription factor AP-2-alpha protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chicken
Synthetic peptide corresponding to Human Transcription factor AP-2-alpha.
Database link: P05549
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab52222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
FunctionSequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle.
Involvement in diseaseDefects in TFAP2A are the cause of branchiooculofacial syndrome (BOFS) [MIM:113620]; also known as branchial clefts with characteristic facies, growth retardation, imperforate nasolacrimal duct, and premature aging or lip pseudocleft-hemangiomatous branchial cyst syndrome. BOFS is a rare autosomal dominant cleft palate craniofacial disorder with variable expressivity. The major features include cutaneous anomalies, ocular anomalies, characteristic facial appearance (malformed pinnae, oral clefts), and, less commonly, renal and ectodermal (dental and hair) anomalies.
Sequence similaritiesBelongs to the AP-2 family.
DomainThe WW-binding motif mediates interaction with WWOX.
modificationsSumoylated on Lys-10; which inhibits transcriptional activity.
- Information by UniProt
- Activating enhancer binding protein 2 alpha antibody
- Activating enhancer-binding protein 2-alpha antibody
- Activator protein 2 antibody
ab52222 has been referenced in 15 publications.
- Xu J et al. miR-876-5p suppresses breast cancer progression through targeting TFAP2A. Exp Ther Med 18:1458-1464 (2019). PubMed: 31316633
- Chen XF et al. An Osteoporosis Risk SNP at 1p36.12 Acts as an Allele-Specific Enhancer to Modulate LINC00339 Expression via Long-Range Loop Formation. Am J Hum Genet 102:776-793 (2018). PubMed: 29706346
- Scott CC et al. TFAP2 transcription factors are regulators of lipid droplet biogenesis. Elife 7:N/A (2018). PubMed: 30256193
- Zhu Z et al. DNA hypomethylation of a transcription factor binding site within the promoter of a gout risk geneNRBP1upregulates its expression by inhibition of TFAP2A binding. Clin Epigenetics 9:99 (2017). PubMed: 28932319
- Balakrishnan A et al. Temporal Analysis of Gene Expression in the Murine Schwann Cell Lineage and the Acutely Injured Postnatal Nerve. PLoS One 11:e0153256 (2016). IHC . PubMed: 27058953