Anti-Transcription factor AP-2-alpha antibody (ab52222)
Key features and details
- Rabbit polyclonal to Transcription factor AP-2-alpha
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Transcription factor AP-2-alpha antibody
See all Transcription factor AP-2-alpha primary antibodies -
Description
Rabbit polyclonal to Transcription factor AP-2-alpha -
Host species
Rabbit -
Specificity
ab52222 detects endogenous levels of total Transcription factor AP-2-alpha protein. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Transcription factor AP-2-alpha.
Database link: P05549 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Chicken
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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| Notes |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
Target
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Function
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. -
Involvement in disease
Defects in TFAP2A are the cause of branchiooculofacial syndrome (BOFS) [MIM:113620]; also known as branchial clefts with characteristic facies, growth retardation, imperforate nasolacrimal duct, and premature aging or lip pseudocleft-hemangiomatous branchial cyst syndrome. BOFS is a rare autosomal dominant cleft palate craniofacial disorder with variable expressivity. The major features include cutaneous anomalies, ocular anomalies, characteristic facial appearance (malformed pinnae, oral clefts), and, less commonly, renal and ectodermal (dental and hair) anomalies. -
Sequence similarities
Belongs to the AP-2 family. -
Domain
The WW-binding motif mediates interaction with WWOX. -
Post-translational
modificationsSumoylated on Lys-10; which inhibits transcriptional activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395982 Chicken
- Entrez Gene: 7020 Human
- Omim: 107580 Human
- SwissProt: P05549 Human
- Unigene: 519880 Human
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Alternative names
- Activating enhancer binding protein 2 alpha antibody
- Activating enhancer-binding protein 2-alpha antibody
- Activator protein 2 antibody
see all
Images
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Western blot - Anti-Transcription factor AP-2-alpha antibody (ab52222)All lanes : Anti-Transcription factor AP-2-alpha antibody (ab52222) at 1/500 dilution
Lane 1 : COLO 205 (Human colon adenocarcinoma cell line) extracts
Lane 2 : COLO 205 (Human colon adenocarcinoma cell line) extracts with immunizing peptide
Predicted band size: 48 kDa
Observed band size: 48 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab52222 has been referenced in 16 publications.
- Mao L et al. Histone Deacetylase 11 Contributes to Renal Fibrosis by Repressing KLF15 Transcription. Front Cell Dev Biol 8:235 (2020). PubMed: 32363192
- Xu J et al. miR-876-5p suppresses breast cancer progression through targeting TFAP2A. Exp Ther Med 18:1458-1464 (2019). PubMed: 31316633
- Chen XF et al. An Osteoporosis Risk SNP at 1p36.12 Acts as an Allele-Specific Enhancer to Modulate LINC00339 Expression via Long-Range Loop Formation. Am J Hum Genet 102:776-793 (2018). PubMed: 29706346
- Scott CC et al. TFAP2 transcription factors are regulators of lipid droplet biogenesis. Elife 7:N/A (2018). PubMed: 30256193
- Zhu Z et al. DNA hypomethylation of a transcription factor binding site within the promoter of a gout risk geneNRBP1upregulates its expression by inhibition of TFAP2A binding. Clin Epigenetics 9:99 (2017). PubMed: 28932319