Anti-GNAT1 antibody (ab3504)
Key features and details
- Rabbit polyclonal to GNAT1
- Suitable for: WB
- Reacts with: Sheep
- Isotype: IgG
Overview
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Product name
Anti-GNAT1 antibody
See all GNAT1 primary antibodies -
Description
Rabbit polyclonal to GNAT1 -
Host species
Rabbit -
Specificity
Detects Rod Transducin alpha from bovine outer rod segments and sheep retinal extract. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Sheep
Predicted to work with: Rat, Dog, Human, Xenopus laevis -
Immunogen
Synthetic peptide corresponding to Human GNAT1 aa 1-100.
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Positive control
- WB: sheep retinal extracts
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa). |
Target
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Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. -
Tissue specificity
Rod. Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium. -
Involvement in disease
Night blindness, congenital stationary, autosomal dominant 3
Night blindness, congenital stationary, 1C -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
Developmental stage
Expressed at approximately postnatal day 7. - Information by UniProt
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Database links
- Entrez Gene: 403613 Dog
- Entrez Gene: 2779 Human
- Entrez Gene: 363143 Rat
- Entrez Gene: 399262 Xenopus laevis
- Omim: 139330 Human
- SwissProt: Q28300 Dog
- SwissProt: P11488 Human
- SwissProt: P38407 Xenopus laevis
see all -
Alternative names
- CSNBAD3 antibody
- GBT1 antibody
- GNAT1 antibody
see all
Datasheets and documents
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Datasheet download
References (3)
ab3504 has been referenced in 3 publications.
- Menad R et al. G protein-coupled oestrogen receptor 1, oestrogen receptors and androgen receptor in the sand rat (Psammomys obesus) efferent ducts. Folia Morphol (Warsz) 79:756-766 (2020). PubMed: 32459366
- Taniuchi K et al. Overexpression of GalNAc-transferase GalNAc-T3 promotes pancreatic cancer cell growth. Oncogene 30:4843-54 (2011). PubMed: 21625220
- Adamus G et al. Molecular biomarkers for autoimmune retinopathies: significance of anti-transducin-alpha autoantibodies. Exp Mol Pathol 87:195-203 (2009). PubMed: 19744478