Product nameAnti-Transferrin antibody [11D3] (HRP)
See all Transferrin primary antibodies
DescriptionMouse monoclonal [11D3] to Transferrin (HRP)
Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
Placental transferrin receptor or transferrin.
- human brain
General notesConcentration varies from lot to lot and can be provided on request.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.05% Proclin
Constituents: PBS, pH 7.4
Concentration information loading...
PurityProtein A purified
Purification notesPurified by chromatography on protein A sepharose. Purity is tested by electrophoresis.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab10208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|Sandwich ELISA||Use at an assay dependent dilution. Can be used as both coating and conjugate antibody when paired with recommended pair for detection of transferrin.|
|WB||1/500. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).|
FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains.
- Information by UniProt
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
All lanes : Anti-Transferrin antibody [11D3] (HRP) (ab10208) at 1/500 dilution
All lanes : human brain
Lysates/proteins at 20 µg per lane.
All lanes : DAKO Cytomation
Conjugation: HRP at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Additional bands at: many <77 kDa (possible non-specific binding)
Exposure time: 1 minute
This product has been referenced in:
- Arulmoli J et al. Combination scaffolds of salmon fibrin, hyaluronic acid, and laminin for human neural stem cell and vascular tissue engineering. Acta Biomater 43:122-138 (2016). Read more (PubMed: 27475528) »
- Shakor AB et al. Sphingomyelin Synthase 1-generated Sphingomyelin Plays an Important Role in Transferrin Trafficking and Cell Proliferation. J Biol Chem 286:36053-62 (2011). Read more (PubMed: 21856749) »