Anti-Transferrin antibody [1F10] (ab218020)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [1F10] (ab218020)](https://www.abcam.com/ps/products/218/ab218020/Images/ab218020-272900-ab218020IHC1.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [1F10] (ab218020)")
Key features and details
- Mouse monoclonal [1F10] to Transferrin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Transferrin antibody [1F10]
See all Transferrin primary antibodies -
Description
Mouse monoclonal [1F10] to Transferrin -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Transferrin aa 1 to the C-terminus. Purified from Human plasma.
Database link: P02787 -
Positive control
- Human liver cancer tissue, Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Aqueous buffered solution -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1F10 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
Sequence similarities
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7018 Human
- Omim: 190000 Human
- SwissProt: P02787 Human
- Unigene: 518267 Human
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Alternative names
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [1F10] (ab218020)
Immunohistochemical analysis of paraformaldehyde fixed, paraffin embedded Human liver cancer tissue labeling Transferrin with ab218020 at 1/400 dilution, followed by a conjugated secondary antibody and DAB staining. Antigen retrieval was by boiling in sodium citrate buffer (pH 6.0) for 15 minutes; block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; blocking buffer (normal goat serum) at 37°C for 30 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [1F10] (ab218020)](https://www.abcam.com/ps/products/218/ab218020/Images/ab218020-272901-ab218020IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [1F10] (ab218020)Immunohistochemical analysis of paraformaldehyde fixed, paraffin embedded Human kidney tissue labeling Transferrin with ab218020 at 1/400 followed by a conjugated secondary antibody and DAB staining. Antigen retrieval was by boiling in sodium citrate buffer (pH 6.0) for 15 minutes; block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; blocking buffer (normal goat serum) at 37°C for 30 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab218020 has not yet been referenced specifically in any publications.
