Overview

  • Product name

    Anti-Transferrin antibody [1F10]
    See all Transferrin primary antibodies
  • Description

    Mouse monoclonal [1F10] to Transferrin
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Transferrin aa 20-698. Purified from Human plasma.
    Database link: P02787

  • Positive control

    • Human liver cancer tissue, Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab218020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • Sequence similarities

    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apotransferrin antibody
    • Beta 1 metal binding globulin antibody
    • Beta-1 metal-binding globulin antibody
    • DKFZp781D0156 antibody
    • PRO1400 antibody
    • PRO1557 antibody
    • PRO2086 antibody
    • Serotransferrin antibody
    • Serotransferrin precursor antibody
    • Siderophilin antibody
    • TF antibody
    • TFQTL1 antibody
    • Transferin antibody
    • Transferrin antibody
    • TRFE_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraformaldehyde fixed, paraffin embedded Human liver cancer tissue labeling Transferrin with ab218020 at 1/400 dilution, followed by a conjugated secondary antibody and DAB staining. Antigen retrieval was by boiling in sodium citrate buffer (pH 6.0) for 15 minutes; block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; blocking buffer (normal goat serum) at 37°C for 30 minutes.

  • Immunohistochemical analysis of paraformaldehyde fixed, paraffin embedded Human kidney tissue labeling Transferrin with ab218020 at 1/400 followed by a conjugated secondary antibody and DAB staining. Antigen retrieval was by boiling in sodium citrate buffer (pH 6.0) for 15 minutes; block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; blocking buffer (normal goat serum) at 37°C for 30 minutes.

References

ab218020 has not yet been referenced specifically in any publications.

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