Anti-Transferrin antibody (ab19177)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Transferrin antibody
See all Transferrin primary antibodies -
DescriptionGoat polyclonal to Transferrin
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Host speciesGoat
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Tested applicationsSuitable for: IHC-P, ELISA, WB, ICCmore details
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Species reactivityReacts with: Human
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Immunogen
Full length native protein (purified) (Human).
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Positive control
- IHC-P: human tissue liver sections
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Affibody® Molecule
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab19177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ELISA | 1/100 - 1/1000. 1000 - 1/30000 (primary) | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 77 kDa. 1/1000 - 1/30000 (Chemiluminescent detection) | |
| ICC | 1/200 - 1/2000. |
Target
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FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
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Tissue specificityExpressed by the liver and secreted in plasma.
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Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
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Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 7018 Human
- Omim: 190000 Human
- SwissProt: P02787 Human
- Unigene: 518267 Human
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Alternative names
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody (ab19177)IHC image of Transferrin staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19177, 1µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References
ab19177 has not yet been referenced specifically in any publications.
