Product nameAnti-Transferrin antibody
See all Transferrin primary antibodies
DescriptionSheep polyclonal to Transferrin
Tested applicationsSuitable for: WB, Double Immunodiffusion, RID, Immunoelectrophoresismore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Guinea pig, Cat, Human
Does not react with: Horse, Chicken, Cow, Dog, Pig
Human holo-transferrin purified from serum and shown to be homogeneous by SDS-PAGE.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.1% EACA, 0.01% Benzamidine, 0.0292% EDTA
Concentration information loading...
Purification notesAntiserum is prepared by immunisation of sheep with Human Holo-Transferrin and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The product is 0.2µm filtered.
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RID and Rocket IEP: 1.5µL antiserum/cm² gel vs 5µL 1/5 – 1/50 dilution human serum
Double diffusion: 10µL antiserum vs 5µL serum
IEP: 100µL antiserum vs 5µL serum.
Optimal dilutions should be determined by the individual laboratory
The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily beexcluded.
FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains.
- Information by UniProt
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
This product has been referenced in:
- Pridans C et al. Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus. J Immunol 201:2683-2699 (2018). Read more (PubMed: 30249809) »
- Murphy S et al. Proteomic profiling of mdx-4cv serum reveals highly elevated levels of the inflammation-induced plasma marker haptoglobin in muscular dystrophy. Int J Mol Med 39:1357-1370 (2017). Read more (PubMed: 28440464) »