Overview

  • Product name

  • Description

    Sheep polyclonal to Transferrin
  • Host species

    Sheep
  • Tested applications

    Suitable for: WB, Double Immunodiffusion, RID, Immunoelectrophoresismore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Cat, Human
    Does not react with: Horse, Chicken, Cow, Dog, Pig
  • Immunogen

    Human holo-transferrin purified from serum and shown to be homogeneous by SDS-PAGE.

Properties

Applications

Our Abpromise guarantee covers the use of ab9033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Double Immunodiffusion
RID
Immunoelectrophoresis
Rocket Immunoelectrophoresis
  • Application notes
    WB: Use at an assay dependent dilution (from PMID 17611631)
    RID and Rocket IEP: 1.5µL antiserum/cm² gel vs 5µL 1/5 – 1/50 dilution human serum
    Double diffusion: 10µL antiserum vs 5µL serum
    IEP: 100µL antiserum vs 5µL serum.

    Optimal dilutions should be determined by the individual laboratory
    The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
    Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily beexcluded.
  • Target

    • Function

      Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
    • Tissue specificity

      Expressed by the liver and secreted in plasma.
    • Involvement in disease

      Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
    • Sequence similarities

      Belongs to the transferrin family.
      Contains 2 transferrin-like domains.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Apotransferrin antibody
      • Beta 1 metal binding globulin antibody
      • Beta-1 metal-binding globulin antibody
      • DKFZp781D0156 antibody
      • PRO1400 antibody
      • PRO1557 antibody
      • PRO2086 antibody
      • Serotransferrin antibody
      • Serotransferrin precursor antibody
      • Siderophilin antibody
      • TF antibody
      • TFQTL1 antibody
      • Transferin antibody
      • Transferrin antibody
      • TRFE_HUMAN antibody
      see all

    References

    This product has been referenced in:

    • Pridans C  et al. Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus. J Immunol 201:2683-2699 (2018). Read more (PubMed: 30249809) »
    • Murphy S  et al. Proteomic profiling of mdx-4cv serum reveals highly elevated levels of the inflammation-induced plasma marker haptoglobin in muscular dystrophy. Int J Mol Med 39:1357-1370 (2017). Read more (PubMed: 28440464) »
    See all 5 Publications for this product

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