Key features and details
- Rabbit polyclonal to Transferrin (Biotin)
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-Transferrin antibody (Biotin)
See all Transferrin primary antibodies
DescriptionRabbit polyclonal to Transferrin (Biotin)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Transferrin from Human serum.
10-20 Biotin molecules per rabbit IgG molecule.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.
Our Abpromise guarantee covers the use of ab34673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 77 kDa.|
FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains.
- Information by UniProt
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
Multiplex western blot analysis of 35 µg of human serum with 350 ng of added GST. Green bands are labelling human transferrin, with ab34673 and a donkey anti-rabbit DyLight® 549 conjugate. Red bands are labelling trypsin, with ab7633 and a donkey anti-goat DyLight® 649 conjugate. Blue bands are labelling human IgG with a primary and a donkey anti-mouse DyLight® 488 conjugate. All primaries were diluted to 1/1,000 and incubated overnight at 4°C. All secondaries were diluted to 1/10,000 for 30 minutes at 4°C.
Anti-Transferrin antibody (Biotin) (ab34673) at 1/1000 dilution + Human depleted serum with Blocking buffer
Peroxidase streptavidin conjugate at 1/40000 dilution
Performed under reducing conditions.
Predicted band size: 77 kDa
Multiplex western blot analysis of human serum. Green bands are labelling human transferrin, with ab34673 and a DyLight® 549 conjugate. Red bands are labelling trypsin, with ab7633 and a DyLight® 649 conjugate. Blue bands are labelling human IgG with a primary and a DyLight® 488 conjugate. All primaries were diluted to 1/1,000 and incubated overnight at 4°C. All secondaries were diluted to 1/10,000 for 1 hour at 4°C.
ab34673 has not yet been referenced specifically in any publications.