Recombinant Anti-Transferrin antibody [EPR18240-29] (ab278498)
![Western blot - Anti-Transferrin antibody [EPR18240-29] (ab278498)](https://www.abcam.com/ps/products/278/ab278498/Images/ab278498-449859-AntiTransferrin-antibodyWB1.jpg "Western blot - Anti-Transferrin antibody [EPR18240-29] (ab278498)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18240-29] to Transferrin
- Suitable for: WB, ICC, Flow Cyt (Intra)
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Transferrin antibody [EPR18240-29]
See all Transferrin primary antibodies -
Description
Rabbit monoclonal [EPR18240-29] to Transferrin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, Flow Cyt (Intra)more details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse liver, kidney, testis tissue lysate and plasma; Hepa1-6 whole cell lysate ICC: Hepa1-6 cells. Flow Cyt (intra): Hepa1-6 (Mouse hepatoma epithelial cells)
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General notes
Please note: Clone [EPR18240-29] (this product) is different to that of ab277635 [EPR20149-224] for the same target.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18240-29 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Dyes/Markers
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab278498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 77 kDa.
|
|
| ICC |
1/100.
|
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| Flow Cyt (Intra) |
1/500.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 77 kDa. |
|
ICC
1/100. |
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Flow Cyt (Intra)
1/500. |
Target
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Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
Sequence similarities
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 22041 Mouse
- SwissProt: Q921I1 Mouse
- Unigene: 37214 Mouse
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Alternative names
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
see all
Images
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Western blot - Anti-Transferrin antibody [EPR18240-29] (ab278498)All lanes : Anti-Transferrin antibody [EPR18240-29] (ab278498) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 77 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1-3: 3.25 seconds; Lane 4: 70 seconds
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![Western blot - Anti-Transferrin antibody [EPR18240-29] (ab278498)](https://www.abcam.com/ps/products/278/ab278498/Images/ab278498-449860-AntiTransferrin-antibodyWB2.jpg)
Western blot - Anti-Transferrin antibody [EPR18240-29] (ab278498)Anti-Transferrin antibody [EPR18240-29] (ab278498) at 1/1000 dilution + Mouse plasma at 20 µl
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 77 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds.
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![Immunocytochemistry - Anti-Transferrin antibody [EPR18240-29] (ab278498)](https://www.abcam.com/ps/products/278/ab278498/Images/ab278498-449861-AntiTransferrin-antibodyICC1.jpg)
Immunocytochemistry - Anti-Transferrin antibody [EPR18240-29] (ab278498)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 cells labelling Transferrin with ab278498 at 1/100 (5.52 μg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Hepa1-6 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-Transferrin antibody [EPR18240-29] (ab278498)](https://www.abcam.com/ps/products/278/ab278498/Images/ab278498-449862-AntiTransferrin-antibodyFC1.jpg)
Flow Cytometry (Intracellular) - Anti-Transferrin antibody [EPR18240-29] (ab278498)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (Mouse hepatoma epithelial cell) cells labelling Transferrin with ab278498 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab278498 has been referenced in 1 publication.
- Tan S et al. Fas/FasL mediates NF-?Bp65/PUMA-modulated hepatocytes apoptosis via autophagy to drive liver fibrosis. Cell Death Dis 12:474 (2021). PubMed: 33980818
