Anti-Transferrin antibody [HTF-14] (ab769)
Key features and details
- Mouse monoclonal [HTF-14] to Transferrin
- Suitable for: ELISA, RIA, ICC, WB, Functional Studies, Sandwich ELISA, IP, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Transferrin antibody [HTF-14]
See all Transferrin primary antibodies -
Description
Mouse monoclonal [HTF-14] to Transferrin -
Host species
Mouse -
Specificity
This antibody recognizes an epitope located in the N-terminal domain of human transferrin. -
Tested applications
Suitable for: ELISA, RIA, ICC, WB, Functional Studies, Sandwich ELISA, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, PigDoes not react with: Sheep, Horse, Cow, Dog, Carp -
Immunogen
Full length native protein (purified) (Pig).
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Positive control
- WB: Human transferrin. IHC-P: Human Placenta
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Purified from ascites by sequential precipitation methods. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
HTF-14 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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RIA |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
Works best under non reducing conditions. |
Functional Studies |
Use at an assay dependent concentration.
Effectively blocks the transferrin-transferrin receptor interactions. |
|
Sandwich ELISA |
Use a concentration of 1 µg/ml.
For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9538 as Detection. |
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 10 µg/ml.
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Notes |
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ELISA
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Works best under non reducing conditions. |
Functional Studies
Use at an assay dependent concentration. Effectively blocks the transferrin-transferrin receptor interactions. |
Sandwich ELISA
Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9538 as Detection. |
IP
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
Sequence similarities
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7018 Human
- Entrez Gene: 100009267 Rabbit
- Omim: 190000 Human
- SwissProt: P02787 Human
- SwissProt: P09571 Pig
- SwissProt: P19134 Rabbit
- Unigene: 518267 Human
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Alternative names
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
see all
Images
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All lanes : Anti-Transferrin antibody [HTF-14] (ab769)
Lane 1 : Human Transferrin – 8 µl (reducing conditions) at 5 µg
Lane 2 : HumanTransferrin – 5 µl (reducing conditions) at 3 µg
Lane 3 : Human Transferrin – 3 µl (reducing conditions) at 1 µg
Lane 4 : Human Transferrin – 3 µl (non reducing conditions) at 1 µg
Lane 5 : Human Transferrin – 5 µl (non reducing conditions) at 3 µg
Lane 6 : Human Transferrin - 8 µl (non reducing conditions) at 5 µgThe sample (commercial human transferrin) (1µg/ml in 1x PBS) was mixed with 4x loading buffer (non reducing) or with Laemmli buffer (reducing) and warmed for 3 minutes.
Volume of sample:5; 3; 1 µl of sample + 3; 2; 2 µl of buffer
SDS Page: 12% AA-gel, U=180V, 90 minutes, volume of loading sample:8; 5;3 µl
Marker – 3 µl
WB: Nitrocelulose membrane (8x5.5 cm), I=35mA, 90minutes
Detection:
Blocking solution: 1xPBS/5% BSA, 4°C, overnight
Primary Ab: membrane was incubated in 5 ml DMEM/10%FTS + 10 µl HTF-14 (primary Ab) (60 minutes, room temperature)
Washing solution: 1x PBS/0.1% Tween
Secondary Ab: IRDye® 800CW Goat anti-mouse IgG1-specific, dilution 1/5000, diluting solution – 5% BSA/1x PBS, incubation for 60 minutes, low temperature.
Detection: Li-COR odyssey
No reducing agent should be added to the sample buffer. The sample can be denatured and processed by common Western blotting protocols, but the disulfide bonds must not be reduced.
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Immunohistochemistry staining of human placenta (paraffin sections) using ab769 10 μg/ml
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab769 has been referenced in 11 publications.
- Zhang LJ et al. Age-Related Loss of Innate Immune Antimicrobial Function of Dermal Fat Is Mediated by Transforming Growth Factor Beta. Immunity 50:121-136.e5 (2019). PubMed: 30594464
- Gianneli M et al. Label-free in-flow detection of receptor recognition motifs on the biomolecular corona of nanoparticles. Nanoscale 10:5474-5481 (2018). PubMed: 29511756
- Lu R et al. Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7. EBioMedicine 36:29-38 (2018). PubMed: 30243490
- Griveau A et al. A Glial Signature and Wnt7 Signaling Regulate Glioma-Vascular Interactions and Tumor Microenvironment. Cancer Cell 33:874-889.e7 (2018). PubMed: 29681511
- Lo Giudice MC et al. In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry. Nat Commun 7:13475 (2016). Flow Cyt ; Human . PubMed: 27845346