Overview

  • Product name
    Anti-Transferrin antibody [HTF-14]
    See all Transferrin primary antibodies
  • Description
    Mouse monoclonal [HTF-14] to Transferrin
  • Host species
    Mouse
  • Specificity
    This antibody recognizes an epitope located in the N-terminal domain of human transferrin.
  • Tested applications
    Suitable for: IHC-P, ELISA, RIA, WB, Sandwich ELISA, ICC, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Rabbit, Human, Pig
    Does not react with: Sheep, Horse, Cow, Dog, Carp
  • Immunogen

    Full length native protein (purified) (Pig).

Properties

Applications

Our Abpromise guarantee covers the use of ab769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Works best under non reducing conditions.

Sandwich ELISA Use a concentration of 1 µg/ml.

For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9538 as Detection.

ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. Effectively blocks the transferrin-transferrin receptor interactions.

Target

  • Function
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • Sequence similarities
    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apotransferrin antibody
    • Beta 1 metal binding globulin antibody
    • Beta-1 metal-binding globulin antibody
    • DKFZp781D0156 antibody
    • PRO1400 antibody
    • PRO1557 antibody
    • PRO2086 antibody
    • Serotransferrin antibody
    • Serotransferrin precursor antibody
    • Siderophilin antibody
    • TF antibody
    • TFQTL1 antibody
    • Transferin antibody
    • Transferrin antibody
    • TRFE_HUMAN antibody
    see all

Images

  • All lanes : Anti-Transferrin antibody [HTF-14] (ab769)

    Lane 1 : Transferrin – 8 µl (5 µg) (reducing conditions)
    Lane 2 : Transferrin – 5 µl (3 µg) (reducing conditions)
    Lane 3 : Transferrin – 3 µl (1 µg) (reducing conditions)
    Lane 4 : Transferrin – 3 µl (1 µg) (non reducing conditions)
    Lane 5 : Transferrin – 5 µl (3 µg) (non reducing conditions)
    Lane 6 : Transferrin - 8 µl (8 µg) (non reducing conditions)


    The sample (commercial human transferrin) (1µg/ml in 1x PBS) was mixed with 4x loading buffer (non reducing) or with Laemmli buffer (reducing) and warmed for 3 minutes.

    Volume of sample:5; 3; 1 µl of sample + 3; 2; 2 µl of buffer

    SDS Page: 12% AA-gel, U=180V, 90 minutes, volume of loading sample:8; 5;3 µl

    Marker – 3 µl

    WB: Nitrocelulose membrane (8x5.5 cm), I=35mA, 90minutes

    Detection:

    Blocking solution: 1xPBS/5% BSA, 4°C, overnight

    Primary Ab: membrane was incubated in 5 ml DMEM/10%FTS + 10 µl HTF-14 (primary Ab) (60 minutes, room temperature)

    Washing solution: 1x PBS/0.1% Tween

    Secondary Ab: IRDye®800CW Goat anti-mouse IgG1-specific, dilution 1/5000, diluting solution – 5% BSA/1x PBS, incubation for 60 minutes, low temperature.

    Detection: Li-COR odyssey

    No reducing agent should be added to the sample buffer. The sample can be denatured and processed by common Western blotting protocols, but the disulfide bonds must not be reduced.

  • Standard curve for Transferrin (Analyte: ab83560); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HTF-14] to Transferrin (ab769) at 1µg/ml and Detector Antibody Rabbit polyclonal to Transferrin (ab9538) at 0.5µg/ml.

References

This product has been referenced in:
  • Lo Giudice MC  et al. In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry. Nat Commun 7:13475 (2016). Flow Cyt ; Human . Read more (PubMed: 27845346) »
  • Shi R  et al. Identification and functional study of osteosarcoma metastasis marker genes. Oncol Lett 10:1848-1852 (2015). WB ; Human . Read more (PubMed: 26622763) »
See all 7 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Lateral flow is not a tested application, however it is very much like sandwich ELISA so any ab pair used in sELISA should work in LF. We do offer a matched antibody for use with ab769 this is ab9538. Gold conjugation is generally used in LF. We do not carry gold conjugated anti-Transferrin, so the customer will need to conjugate one ab for use as the colored marker. 1) Ab9538 is tested and guaranteed in sELISA. For use in LT we recommend using this ab9583 as the test line and ab769 as colored particle. We do not have a gold conjugation kit however these may be found at online. Control line will be Mouse IgG1 monoclonal antibody [NCM1] - Isotype control (ab27479). You will need a detection system which will be able to read the gold particles, a flatbed scanner will likely work. 2) We do not test in LT. Ab769 is known to Reacts with: Rabbit, Human, Pig and not with Sheep, Horse, Cow, Dog or Carp. AB9538 is known to react with human and Marmoset; other species have not been tested. 3) Transferrin protein (Active) ab83560 has been tested with and is the positive control for sELISA with this pair.

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Question
Answer

Thank you for contacting Abcam.

Since all three antibodies have IgG (or a IgG subclass) isotypes, the molecular weight of each is approximately 160kDa (23-25kDa for both light chains, and 53kDa for both heavy chains).

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Application
Western blot
Sample
Human Tissue lysate - other (artery biopsy)
Loading amount
20 µg
Specification
artery biopsy
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Abcam user community

Verified customer

Submitted Feb 06 2007

Answer

Thank you for the requested information. I have re-examined our record and I was not able to find this information in our past correspondences with you. In regards to the purchase order number "192677", this was an order for ab7355 (Acinus antibody) placed by Patricia from the Inserm. Could you please check this information again and provide the correct PO# for ab769? Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement if the product has been purchased within 90 days. Again, I apologize for any inconveniences and I look forward to your reply.

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