Product nameAnti-Transferrin antibody [HTF-14]
See all Transferrin primary antibodies
DescriptionMouse monoclonal [HTF-14] to Transferrin
SpecificityThis antibody recognizes an epitope located in the N-terminal domain of human transferrin.
Tested applicationsSuitable for: IHC-P, ELISA, RIA, WB, Sandwich ELISA, ICC, IP, Functional Studiesmore details
Species reactivityReacts with: Rabbit, Human, Pig
Does not react with: Sheep, Horse, Cow, Dog, Carp
Full length native protein (purified) (Pig).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
Purification notesPurified from ascites by sequential precipitation methods. Purity >95% by SDS-PAGE.
Light chain typeunknown
Our Abpromise guarantee covers the use of ab769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.
Works best under non reducing conditions.
|Sandwich ELISA||Use a concentration of 1 µg/ml.
For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9538 as Detection.
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration. Effectively blocks the transferrin-transferrin receptor interactions.|
FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains.
- Information by UniProt
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
All lanes : Anti-Transferrin antibody [HTF-14] (ab769)
Lane 1 : Transferrin – 8 µl (5 µg) (reducing conditions)
Lane 2 : Transferrin – 5 µl (3 µg) (reducing conditions)
Lane 3 : Transferrin – 3 µl (1 µg) (reducing conditions)
Lane 4 : Transferrin – 3 µl (1 µg) (non reducing conditions)
Lane 5 : Transferrin – 5 µl (3 µg) (non reducing conditions)
Lane 6 : Transferrin - 8 µl (8 µg) (non reducing conditions)
The sample (commercial human transferrin) (1µg/ml in 1x PBS) was mixed with 4x loading buffer (non reducing) or with Laemmli buffer (reducing) and warmed for 3 minutes.
Volume of sample:5; 3; 1 µl of sample + 3; 2; 2 µl of buffer
SDS Page: 12% AA-gel, U=180V, 90 minutes, volume of loading sample:8; 5;3 µl
Marker – 3 µl
WB: Nitrocelulose membrane (8x5.5 cm), I=35mA, 90minutes
Blocking solution: 1xPBS/5% BSA, 4°C, overnight
Primary Ab: membrane was incubated in 5 ml DMEM/10%FTS + 10 µl HTF-14 (primary Ab) (60 minutes, room temperature)
Washing solution: 1x PBS/0.1% Tween
Secondary Ab: IRDye®800CW Goat anti-mouse IgG1-specific, dilution 1/5000, diluting solution – 5% BSA/1x PBS, incubation for 60 minutes, low temperature.
Detection: Li-COR odyssey
No reducing agent should be added to the sample buffer. The sample can be denatured and processed by common Western blotting protocols, but the disulfide bonds must not be reduced.
This product has been referenced in:
- Lu R et al. Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7. EBioMedicine 36:29-38 (2018). Read more (PubMed: 30243490) »
- Lo Giudice MC et al. In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry. Nat Commun 7:13475 (2016). Flow Cyt ; Human . Read more (PubMed: 27845346) »