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We wish to create a lateral flow assay, I am shopping for antibodies against human transferrin. Please help me on following questions. 1) Antibody pairing information to generate lateral flow test for detecting human transferrin 2) If such pairing information is available, we would like to know the performance of such pair in lateral flow test, i.e. the sensitivity and specificity (crossreactivity with transferrin from other species). 3) Positive control information to validate the antibody performance in lateral flow test.
Asked on Dec 28 2012
Lateral flow is not a tested application, however it is very much like sandwich ELISA so any ab pair used in sELISA should work in LF. We do offer a matched antibody for use with ab769 this is ab9538. Gold conjugation is generally used in LF. We do not carry gold conjugated anti-Transferrin, so the customer will need to conjugate one ab for use as the colored marker. 1) Ab9538 is tested and guaranteed in sELISA. For use in LT we recommend using this ab9583 as the test line and ab769 as colored particle. We do not have a gold conjugation kit however these may be found at online. Control line will be Mouse IgG1 monoclonal antibody [NCM1] - Isotype control (ab27479). You will need a detection system which will be able to read the gold particles, a flatbed scanner will likely work. 2) We do not test in LT. Ab769 is known to Reacts with: Rabbit, Human, Pig and not with Sheep, Horse, Cow, Dog or Carp. AB9538 is known to react with human and Marmoset; other species have not been tested. 3) Transferrin protein (Active) ab83560 has been tested with and is the positive control for sELISA with this pair.
Answered on Dec 28 2012