Other product details
Host species: Human
Conjugation: Horse Radish Peroxidase
1) Load more sample protein e.g. 50-100ug/lane.
2) Reduce the percentage of blocking agent and the blocking time i.e. block in 5% milk or BSA for 30min at room temperature.
3) Dilute ab769 in PBST/TBST alone (no blocking agents as they might inhibit antibody-antigen binding) and incubate membrane in ab769 overnight at 4C.
4) Dilute the secondary antibody at 1:5000-10,000. At 1:20,000, the secondary might be overdiluted/insufficient.
Please let us know if this improves your results.
Abcam user community
Submitted Feb 06 2007