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    transferrin-antibody-tf3001-bsa-and-azide-free-ab268240.pdf

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Cardiovascular Blood Serum Proteins
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Anti-Transferrin antibody [TF/3001] - BSA and Azide free (ab268240)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [TF/3001] - BSA and Azide free (ab268240)
  • Protein Array - Anti-Transferrin antibody [TF/3001] - BSA and Azide free (ab268240)

Key features and details

  • Mouse monoclonal [TF/3001] to Transferrin - BSA and Azide free
  • Suitable for: IHC-P, Protein Array
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Transferrin antibody [TF/3001] - BSA and Azide free
    See all Transferrin primary antibodies
  • Description

    Mouse monoclonal [TF/3001] to Transferrin - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Protein Arraymore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Transferrin aa 311-445. The exact sequence is proprietary.
    Database link: P02787

  • Positive control

    • IHC-P: Human prostate carcinoma tissue.
  • General notes

    ab268240 is the carrier-free version of ab268117.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from Bioreactor concentrate
  • Clonality

    Monoclonal
  • Clone number

    TF/3001
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cardiovascular
    • Blood
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cardiovascular ELISA kits

Associated products

  • Alternative Versions

    • Anti-Transferrin antibody [TF/3001] (ab268117)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab268240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protein Array
Use at an assay dependent concentration.
Notes
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protein Array
Use at an assay dependent concentration.

Target

  • Function

    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • Sequence similarities

    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02787 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7018 Human
    • Omim: 190000 Human
    • SwissProt: P02787 Human
    • Unigene: 518267 Human
    • Alternative names

      • Apotransferrin antibody
      • Beta 1 metal binding globulin antibody
      • Beta-1 metal-binding globulin antibody
      • DKFZp781D0156 antibody
      • PRO1400 antibody
      • PRO1557 antibody
      • PRO2086 antibody
      • Serotransferrin antibody
      • Serotransferrin precursor antibody
      • Siderophilin antibody
      • TF antibody
      • TFQTL1 antibody
      • Transferin antibody
      • Transferrin antibody
      • TRFE_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [TF/3001] - BSA and Azide free (ab268240)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [TF/3001] - BSA and Azide free (ab268240)

      Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Transferrin using ab268117 at 2 μg/ml in immunohistochemical analysis.

      This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268117).

    • Protein Array - Anti-Transferrin antibody [TF/3001] - BSA and Azide free (ab268240)
      Protein Array - Anti-Transferrin antibody [TF/3001] - BSA and Azide free (ab268240)

      Protein Array containing >19,000 full-length human proteins using ab268117.

      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

      This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268240)

      This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268117).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab268240? Please let us know so that we can cite the reference in this datasheet.

    ab268240 has not yet been referenced specifically in any publications.

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