Anti-Transferrin Receptor 2/TFR2 antibody (ab228796)
Key features and details
- Rabbit polyclonal to Transferrin Receptor 2/TFR2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Transferrin Receptor 2/TFR2 antibody
See all Transferrin Receptor 2/TFR2 primary antibodies -
Description
Rabbit polyclonal to Transferrin Receptor 2/TFR2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Transferrin Receptor 2/TFR2 (internal sequence). The exact sequence is proprietary.
Database link: Q9UP52 -
Positive control
- WB: K562 and HL-60 whole cell lysate. IHC-P: Human hepatoma tissue.
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General notes
This product was previously labelled as Transferrin Receptor 2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab228796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 89 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation. -
Tissue specificity
Predominantly expressed in liver. While the alpha form is also expressed in spleen, lung, muscle, prostate and peripheral blood mononuclear cells, the beta form is expressed in all tissues tested, albeit weakly. -
Involvement in disease
Defects in TFR2 are a cause of hemochromatosis type 3 (HFE3) [MIM:604250]. HFE3 is a disorder of iron hemostasis resulting in iron overload and has a phenotype indistinguishable from that of hereditary hemochromatosis (HH). HH is characterized by abnormal intestinal iron absorption and progressive increase of total body iron, which results in midlife in clinical complications including cirrhosis, cardiopathy, diabetes, endocrine dysfunctions, arthropathy, and susceptibility to liver cancer. Since the disease complications can be effectively prevented by regular phlebotomies, early diagnosis is most important to provide a normal life expectancy to the affected subjects. -
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily. -
Cellular localization
Cell membrane and Cytoplasm. Lacks the transmembrane domain. Probably intracellular. - Information by UniProt
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Database links
- Entrez Gene: 7036 Human
- Omim: 604720 Human
- SwissProt: Q9UP52 Human
- Unigene: 544932 Human
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Alternative names
- HFE 3 antibody
- HFE3 antibody
- MGC126368 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor 2/TFR2 antibody (ab228796)
Paraffin-embedded human hepatoma tissue stained for Transferrin Receptor 2/TFR2 using ab228796 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Transferrin Receptor 2/TFR2 antibody (ab228796)All lanes : Anti-Transferrin Receptor 2/TFR2 antibody (ab228796) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 89 kDa7.5% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab228796 has not yet been referenced specifically in any publications.
