Recombinant
RabMAb

Recombinant Anti-Transferrin Receptor 2/TFR2 antibody [EPR15322] (ab185550)

Overview

  • Product name

    Anti-Transferrin Receptor 2/TFR2 antibody [EPR15322]
    See all Transferrin Receptor 2/TFR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15322] to Transferrin Receptor 2/TFR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Transferrin Receptor 2/TFR2 aa 150-300. The exact sequence is proprietary.
    Database link: Q9UP52

  • Positive control

    • HepG2 and TF-1 cell lysates; Human fetal liver lysate; Human liver tissue.
  • General notes

     

     

     This product was previously labelled as Transferrin Receptor 2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 97-105 kDa (predicted molecular weight: 89 kDa).
IHC-P 1/500.

Target

  • Function

    Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation.
  • Tissue specificity

    Predominantly expressed in liver. While the alpha form is also expressed in spleen, lung, muscle, prostate and peripheral blood mononuclear cells, the beta form is expressed in all tissues tested, albeit weakly.
  • Involvement in disease

    Defects in TFR2 are a cause of hemochromatosis type 3 (HFE3) [MIM:604250]. HFE3 is a disorder of iron hemostasis resulting in iron overload and has a phenotype indistinguishable from that of hereditary hemochromatosis (HH). HH is characterized by abnormal intestinal iron absorption and progressive increase of total body iron, which results in midlife in clinical complications including cirrhosis, cardiopathy, diabetes, endocrine dysfunctions, arthropathy, and susceptibility to liver cancer. Since the disease complications can be effectively prevented by regular phlebotomies, early diagnosis is most important to provide a normal life expectancy to the affected subjects.
  • Sequence similarities

    Belongs to the peptidase M28 family. M28B subfamily.
  • Cellular localization

    Cell membrane and Cytoplasm. Lacks the transmembrane domain. Probably intracellular.
  • Information by UniProt
  • Database links

  • Alternative names

    • HFE 3 antibody
    • HFE3 antibody
    • MGC126368 antibody
    • TFR 2 antibody
    • TfR2 antibody
    • TFR2_HUMAN antibody
    • TFRC 2 antibody
    • TFRC2 antibody
    • Transferrin receptor 2 antibody
    • Transferrin receptor protein 2 antibody
    see all

Images

  • Anti-Transferrin Receptor 2/TFR2 antibody [EPR15322] (ab185550) at 1/5000 dilution + TF-1 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 89 kDa

  • Anti-Transferrin Receptor 2/TFR2 antibody [EPR15322] (ab185550) at 1/5000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 89 kDa

  • Anti-Transferrin Receptor 2/TFR2 antibody [EPR15322] (ab185550) at 1/20000 dilution + Human fetal liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 89 kDa

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Transferrin Receptor 2/TFR2 with ab185550 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (macrophage)
Permeabilization
Yes - saponin
Specification
macrophage
Blocking step
Gelatin as blocking agent for 10 minute(s) · Concentration: 0.2% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 05 2015

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