Anti-Transferrin Receptor antibody [11F5] (ab10249)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Transferrin Receptor antibody [11F5]
See all Transferrin Receptor primary antibodies -
DescriptionMouse monoclonal [11F5] to Transferrin Receptor
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Host speciesMouse
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SpecificityThis antibody reacts with human soluble transferrin receptor.
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Tested applicationsSuitable for: Sandwich ELISA, ELISAmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant full length protein (Human).
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General notesConcentration varies from lot to lot and can be provided on request.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein A purified
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Purification notesPurity tested by electrophoresis.
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ClonalityMonoclonal
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Clone number11F5
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MyelomaSp2/0
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IsotypeIgG2b
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab10249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | ||
| ELISA |
Application notes
ELISA: Use at an assay dependent dilution.
sELISA: Can be paired for ELISA with Mouse monoclonal [7F8] to Transferrin Receptor (HRP) (ab10254). Can be used as detection antibody with Mouse monoclonal [2B6] to Transferrin Receptor (ab38168) as capture antibody. Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
sELISA: Can be paired for ELISA with Mouse monoclonal [7F8] to Transferrin Receptor (HRP) (ab10254). Can be used as detection antibody with Mouse monoclonal [2B6] to Transferrin Receptor (ab38168) as capture antibody. Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
Involvement in diseaseImmunodeficiency 46
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Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
Post-translational
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 7037 Human
- Omim: 190010 Human
- SwissProt: P02786 Human
- Unigene: 529618 Human
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Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
References
This product has been referenced in:
- Peterson RD et al. Comparison of Methods Study between a Photonic Crystal Biosensor and Certified ELISA to Measure Biomarkers of Iron Deficiency in Chronic Kidney Disease Patients. Sensors (Basel) 17:N/A (2017). Read more (PubMed: 28946680) »
