Key features and details
- Mouse monoclonal [11F5] to Transferrin Receptor
- Suitable for: ELISA, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-Transferrin Receptor antibody [11F5]
See all Transferrin Receptor primary antibodies
DescriptionMouse monoclonal [11F5] to Transferrin Receptor
SpecificityThis antibody reacts with human soluble transferrin receptor.
Tested applicationsSuitable for: ELISA, Sandwich ELISAmore details
Species reactivityReacts with: Human
Recombinant full length protein (Human).
General notesConcentration varies from lot to lot and can be provided on request.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesPurity tested by electrophoresis.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Can be paired for ELISA with Mouse monoclonal [7F8] to Transferrin Receptor (HRP) (ab10254). Can be used as detection antibody with Mouse monoclonal [2B6] to Transferrin Receptor (ab38168) as capture antibody. Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Involvement in diseaseImmunodeficiency 46
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab10249 has been referenced in 2 publications.
- Peterson RD et al. Comparison of Methods Study between a Photonic Crystal Biosensor and Certified ELISA to Measure Biomarkers of Iron Deficiency in Chronic Kidney Disease Patients. Sensors (Basel) 17:N/A (2017). PubMed: 28946680
- Peterson RD et al. Enhanced sandwich immunoassay using antibody-functionalized magnetic iron-oxide nanoparticles for extraction and detection of soluble transferrin receptor on a photonic crystal biosensor. Biosens Bioelectron 74:815-22 (2015). PubMed: 26232676