Anti-Transferrin Receptor antibody (ab166929)

Overview

  • Product name
    Anti-Transferrin Receptor antibody
    See all Transferrin Receptor primary antibodies
  • Description
    Goat polyclonal to Transferrin Receptor
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Chinese hamster
  • Immunogen

    Synthetic peptide with a Cysteine residue linker, corresponding to N terminal amino acids 21-35 of Human Transferrin Receptor (NP_003225.2).

  • Positive control
    • Human Breast lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab166929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 85 kDa.

Target

  • Function
    Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in disease
    Immunodeficiency 46
  • Sequence similarities
    Belongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications
    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localization
    Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • IMD46 antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Images

  • Anti-Transferrin Receptor antibody (ab166929) at 1 µg/ml + Human Breast lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 85 kDa



    Primary incubation was 1 hour.

References

ab166929 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bos taurus Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Melissa Kelly

Verified customer

Submitted Aug 22 2017

Question
Answer

The transferring receptor antibody ab166929 will recognize just the membrane form, as it is raised against the N terminal amino acids 21-35, and the soluble serum form is amino acids 101-760, referring to the numbering used for the UniProt entry at this link: http://www.uniprot.org/uniprot/P02786.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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