Key features and details
- Rabbit polyclonal to Transferrin Receptor
- Suitable for: WB, IHC-P, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Transferrin Receptor antibody
See all Transferrin Receptor primary antibodies
DescriptionRabbit polyclonal to Transferrin Receptor
Tested applicationsSuitable for: WB, IHC-P, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Transferrin Receptor aa 94-754.
PKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGT IKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVK DSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDF EDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNA ELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAE KLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVI KGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQ PSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSN FKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFP FLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEV AGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLY SARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPK ESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQ GAANALSGDV W
Database link: P02786
- WB: U251, 293T and Jurkat whole cell lysates. IHC-P: Human placenta and human colon cancer tissues. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab193188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).|
|IHC-P||1/20 - 1/200.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/200.|
FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Involvement in diseaseImmunodeficiency 46
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
All lanes : Anti-Transferrin Receptor antibody (ab193188) at 2 µg/ml
Lane 1 : U251 whole cell lysate
Lane 2 : 293T whole cell lysate
All lanes : Polyclonal goat anti-rabbit at 1/10000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling Transferrin Receptor with ab193188 at a dilution of 1/100.
Anti-Transferrin Receptor antibody (ab193188) at 3 µg/ml + Jurkat whole cell lysate
Polyclonal goat anti-rabbit IgG at 1/50000 dilution
Predicted band size: 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue labelling Transferrin Receptor with ab193188 at a dilution of 1/100.
Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Transferrin Receptor with ab193188 at a dilution of 1/100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody.
ab193188 has not yet been referenced specifically in any publications.