Key features and details
- Rabbit polyclonal to Transferrin Receptor
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Transferrin Receptor antibody
See all Transferrin Receptor primary antibodies
DescriptionRabbit polyclonal to Transferrin Receptor
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide aa 620-670 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P02786
- IHC-P: Human lung carcinoma tissue. WB: Mouse placenta lysate. ICC/IF: U-138. Flow Cyt: Raji cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/1000. Predicted molecular weight: 84 kDa.|
|ICC/IF||1/50 - 1/200.|
|Flow Cyt||1/20 - 1/100.|
FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Involvement in diseaseImmunodeficiency 46
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
Anti-Transferrin Receptor antibody (ab216665) at 1/300 dilution + mouse placenta lysate
Developed using the ECL technique.
Predicted band size: 84 kDa
Flow cytometry analysis of Raji (Human Burkitt's lymphoma cell line) cells labeling Transferrin Receptor (green, right histogram), using ab216665 at a 1/100 dilution, and negative control cells (blue). As secondary antibody PE-conjugated was used. Secondary only (light blue) and isotope control (orange).
Immunofluorescence analysis of formalin-fixed U-138 (Human glioblastoma cell line) cells, labeling Transferrin Receptor using ab216665 at a 1/50 dilution; followed by secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit lgG (H+L).
Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung carcinoma tissue, labeling Transferrin Receptor using ab216665 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
ab216665 has not yet been referenced specifically in any publications.