Overview

  • Product name

    Anti-Transferrin Receptor antibody
    See all Transferrin Receptor primary antibodies
  • Description

    Rabbit polyclonal to Transferrin Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide aa 620-670 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P02786

  • Positive control

    • IHC-P: Human lung carcinoma tissue. WB: Mouse placenta lysate. ICC/IF: U138. FACS: Raji cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab216665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/1000. Predicted molecular weight: 84 kDa.
ICC/IF 1/50 - 1/200.
Flow Cyt 1/20 - 1/100.

Target

  • Function

    Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in disease

    Immunodeficiency 46
  • Sequence similarities

    Belongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications

    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localization

    Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • IMD46 antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Images

  • Anti-Transferrin Receptor antibody (ab216665) at 1/300 dilution + mouse placenta lysate

    Developed using the ECL technique.

    Predicted band size: 84 kDa

  • Flow cytometry analysis of Raji cells labeling Transferrin Receptor (green, right histogram), using ab216665 at a 1/100 dilution, and negative control cells (blue). As secondary antibody PE-conjugated was used. Secondary only (light blue) and isotope control (orange).

  • Immunofluorescence analysis of formalin-fixed U138 cells, labeling Transferrin Receptor using ab216665 at a 1/50 dilution; followed by secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit lgG (H+L).

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung carcinoma tissue, labeling Transferrin Receptor using ab216665 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

References

ab216665 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva
Permeabilization
No
Specification
Kidney
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 20 2018

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