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Anti-Transferrin Receptor antibody (ab84036)

  • Datasheet
  • SDS
Reviews (22)Q&A (9)References (86)

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Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab84036)
  • Western blot - Anti-Transferrin Receptor antibody (ab84036)
  • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab84036)
  • Western blot - Anti-Transferrin Receptor antibody (ab84036)
  • Flow Cytometry - Anti-Transferrin Receptor antibody (ab84036)

Key features and details

  • Rabbit polyclonal to Transferrin Receptor
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Anti-Transferrin Receptor 2/TFR2 antibody (ab80194)

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Overview

  • Product name

    Anti-Transferrin Receptor antibody
    See all Transferrin Receptor primary antibodies
  • Description

    Rabbit polyclonal to Transferrin Receptor
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Transferrin Receptor aa 1-100 conjugated to keyhole limpet haemocyanin.
    Database link: P02786
    (Peptide available as ab101219)

  • Positive control

    • ICC/IF: HepG2 and HeLa cells, WB: HeLa, Jurkat and U-2 OS whole cell lysates; Mouse spleen tissue lysate. Flow Cyt: MCF7 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Stem Cells
    • Neural Stem Cells
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Erythrocytic Lineage
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Transferrin Receptor peptide (ab101219)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab84036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
WB
Mouse
Human
All applications
Dog
Pig
Orangutan
Application Abreviews Notes
WB (9)
Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).
IHC-P (3)
1/6000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF (6)
Use a concentration of 5 µg/ml.
Flow Cyt (1)
Use 1µg for 106 cells.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).
IHC-P
1/6000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

Target

  • Function

    Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in disease

    Immunodeficiency 46
  • Sequence similarities

    Belongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications

    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localization

    Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P02786 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7037 Human
    • Entrez Gene: 22042 Mouse
    • Entrez Gene: 100171944 Orangutan
    • Omim: 190010 Human
    • SwissProt: P02786 Human
    • SwissProt: Q62351 Mouse
    • SwissProt: Q5RDH6 Orangutan
    • Unigene: 529618 Human
    • Unigene: 28683 Mouse
    see all
  • Alternative names

    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • IMD46 antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab84036)
    Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab84036)D'Hooghe et al PLoS One. 2017 Aug 17;12(8):e0182695. doi: 10.1371/journal.pone.0182695. eCollection 2017. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Analysis of co-localisation of FcRn with cellular markers.

    HepG2 (Human liver hepatocellular carcinoma cell line) cells were fixed, permeabilized and co-stained with anti-FcRn humanised Fab’, followed by anti-human IgG conjugated Alexa Fluor®488 (Top left panel. Bar 5 μm) and ab84036 followed by anti-rabbit/mouse conjugated Alexa Fluor®568 (Top right panel). FcRn fluorescence is shown in green and marker fluorescence is shown in red, yellow fluorescence in the overlay images (Bottom left panel) indicates co-localization.

    Images are magnifications of the white boxed area (Bottom right panel. Bar 20 μm). 

  • Western blot - Anti-Transferrin Receptor antibody (ab84036)
    Western blot - Anti-Transferrin Receptor antibody (ab84036)
    All lanes : Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) whole cell lysate
    Lane 3 : U-2 OS (Human osteosarcoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 84 kDa
    Observed band size: 98 kDa why is the actual band size different from the predicted?
    Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab84036)
    Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab84036)

    ICC/IF image of ab84036 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    The cells were fixed in 4% formaldehyde (10 minutes) then permeabilized using 0.1% PBS-Triton and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to further permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab84036 at 1 µg/ml overnight at +4°C.

    The secondary antibody (pseudo-colored green) was an Alexa-Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1 hour. Alexa-Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature.

    DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43 µM for 1 hour at room temperature.

  • Western blot - Anti-Transferrin Receptor antibody (ab84036)
    Western blot - Anti-Transferrin Receptor antibody (ab84036)
    Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml + Mouse spleen tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 84 kDa
    Observed band size: 100,50 kDa why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Flow Cytometry - Anti-Transferrin Receptor antibody (ab84036)
    Flow Cytometry - Anti-Transferrin Receptor antibody (ab84036)

    Overlay histogram showing MCF7 cells stained with ab84036 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab84036) (1x106 in 100µl at 1 µg/ml) for 30 min at 22ºC.

    The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 22ºC.

    Isotype control antibody (black line) was Rabbit IgG (polyclonal) (ab171870) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (86)

Publishing research using ab84036? Please let us know so that we can cite the reference in this datasheet.

ab84036 has been referenced in 86 publications.

  • Zhao H  et al. Long-term voluntary running prevents the onset of symptomatic Friedreich's ataxia in mice. Sci Rep 10:6095 (2020). WB ; Mouse . PubMed: 32269244
  • Shang Y  et al. Ceruloplasmin suppresses ferroptosis by regulating iron homeostasis in hepatocellular carcinoma cells. Cell Signal 72:109633 (2020). PubMed: 32283255
  • Lee DG  et al. Peroxiredoxin 5 deficiency exacerbates iron overload-induced neuronal death via ER-mediated mitochondrial fission in mouse hippocampus. Cell Death Dis 11:204 (2020). PubMed: 32205843
  • Promchan K & Natarajan V Leucine zipper transcription factor-like 1 binds adaptor protein complex-1 and 2 and participates in trafficking of transferrin receptor 1. PLoS One 15:e0226298 (2020). PubMed: 31895934
  • Singh KS  et al. African-centric TP53 variant increases iron accumulation and bacterial pathogenesis but improves response to malaria toxin. Nat Commun 11:473 (2020). PubMed: 31980600
View all Publications for this product

Customer reviews and Q&As

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1-10 of 31 Abreviews or Q&A

Immunoprecipitation abreview for Anti-Transferrin Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HCT116 cell line)
Total protein in input
1000 µg
Immuno-precipitation step
Other - Dynabeads Protein G
Specification
HCT116 cell line
Read More

Ms. Maria del Carmen Conde Rubio

Verified customer

Submitted May 09 2018

Western blot abreview for Anti-Transferrin Receptor antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Hamster Cell lysate - whole cell (BHKT7)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
250000 cells
Specification
BHKT7
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted May 28 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Transferrin Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (undifferentiated Human ES cells on MEFs)
Permeabilization
Yes - see blocking buffer
Specification
undifferentiated Human ES cells on MEFs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 05 2016

Western blot abreview for Anti-Transferrin Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Hamster Cell lysate - other (Baby hamster kidney fibroblast cells (BHK-21))
Gel Running Conditions
Reduced Denaturing (12 SDS-PAGE)
Loading amount
10 µg
Specification
Baby hamster kidney fibroblast cells (BHK-21)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Feb 29 2016

Western blot abreview for Anti-Transferrin Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (undifferentiated Human ES cells on MEFs)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
20 µg
Specification
undifferentiated Human ES cells on MEFs
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Jan 15 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Transferrin Receptor antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEPG2)
Specification
HEPG2
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 18 2015

Western blot abreview for Anti-Transferrin Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEPG2
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Read More

Abcam user community

Verified customer

Submitted Nov 13 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Transferrin Receptor antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK cells)
Permeabilization
Yes - 0.1% DOC
Specification
MDCK cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

馨 宮原

Verified customer

Submitted Oct 23 2015

Western blot abreview for Anti-Transferrin Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7 and MET5A cancer cell lines)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
100000 cells
Specification
MCF7 and MET5A cancer cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Read More

Abcam user community

Verified customer

Submitted Aug 18 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Transferrin Receptor antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Human Tissue sections (Placenta)
Specification
Placenta
Permeabilization
No
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted Nov 20 2014

1-10 of 31 Abreviews or Q&A

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