Anti-Transferrin Receptor antibody (ab84036)
Key features and details
- Rabbit polyclonal to Transferrin Receptor
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Transferrin Receptor antibody
See all Transferrin Receptor primary antibodies -
Description
Rabbit polyclonal to Transferrin Receptor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB MouseHuman
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Immunogen
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Positive control
- ICC/IF: HepG2 and HeLa cells, WB: HeLa, Jurkat and U-2 OS whole cell lysates; Mouse spleen tissue lysate. Flow Cyt: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
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| ICC/IF |
Human
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| WB |
Mouse
Human
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| All applications |
Dog
Pig
Orangutan
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| Application | Abreviews | Notes |
|---|---|---|
| WB | (9) |
Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).
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| IHC-P | (3) |
1/6000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF | (6) |
Use a concentration of 5 µg/ml.
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| Flow Cyt | (1) |
Use 1µg for 106 cells.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa). |
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IHC-P
1/6000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 5 µg/ml. |
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Flow Cyt
Use 1µg for 106 cells. |
Target
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Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
Involvement in disease
Immunodeficiency 46 -
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
Post-translational
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 7037 Human
- Entrez Gene: 22042 Mouse
- Entrez Gene: 100171944 Orangutan
- Omim: 190010 Human
- SwissProt: P02786 Human
- SwissProt: Q62351 Mouse
- SwissProt: Q5RDH6 Orangutan
- Unigene: 529618 Human
see all -
Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab84036)D'Hooghe et al PLoS One. 2017 Aug 17;12(8):e0182695. doi: 10.1371/journal.pone.0182695. eCollection 2017. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Analysis of co-localisation of FcRn with cellular markers.
HepG2 (Human liver hepatocellular carcinoma cell line) cells were fixed, permeabilized and co-stained with anti-FcRn humanised Fab’, followed by anti-human IgG conjugated Alexa Fluor®488 (Top left panel. Bar 5 μm) and ab84036 followed by anti-rabbit/mouse conjugated Alexa Fluor®568 (Top right panel). FcRn fluorescence is shown in green and marker fluorescence is shown in red, yellow fluorescence in the overlay images (Bottom left panel) indicates co-localization.
Images are magnifications of the white boxed area (Bottom right panel. Bar 20 μm).
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Western blot - Anti-Transferrin Receptor antibody (ab84036)All lanes : Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) whole cell lysate
Lane 3 : U-2 OS (Human osteosarcoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab84036)ICC/IF image of ab84036 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in 4% formaldehyde (10 minutes) then permeabilized using 0.1% PBS-Triton and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to further permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab84036 at 1 µg/ml overnight at +4°C.
The secondary antibody (pseudo-colored green) was an Alexa-Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1 hour. Alexa-Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature.
DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43 µM for 1 hour at room temperature.
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Western blot - Anti-Transferrin Receptor antibody (ab84036)Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml + Mouse spleen tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 100,50 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Flow Cytometry - Anti-Transferrin Receptor antibody (ab84036)Overlay histogram showing MCF7 cells stained with ab84036 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab84036) (1x106 in 100µl at 1 µg/ml) for 30 min at 22ºC.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 22ºC.
Isotype control antibody (black line) was Rabbit IgG (polyclonal) (ab171870) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (86)
ab84036 has been referenced in 86 publications.
- Zhao H et al. Long-term voluntary running prevents the onset of symptomatic Friedreich's ataxia in mice. Sci Rep 10:6095 (2020). WB ; Mouse . PubMed: 32269244
- Shang Y et al. Ceruloplasmin suppresses ferroptosis by regulating iron homeostasis in hepatocellular carcinoma cells. Cell Signal 72:109633 (2020). PubMed: 32283255
- Lee DG et al. Peroxiredoxin 5 deficiency exacerbates iron overload-induced neuronal death via ER-mediated mitochondrial fission in mouse hippocampus. Cell Death Dis 11:204 (2020). PubMed: 32205843
- Promchan K & Natarajan V Leucine zipper transcription factor-like 1 binds adaptor protein complex-1 and 2 and participates in trafficking of transferrin receptor 1. PLoS One 15:e0226298 (2020). PubMed: 31895934
- Singh KS et al. African-centric TP53 variant increases iron accumulation and bacterial pathogenesis but improves response to malaria toxin. Nat Commun 11:473 (2020). PubMed: 31980600