Anti-Transferrin Receptor antibody (ab84036)
Key features and details
- Rabbit polyclonal to Transferrin Receptor
- Suitable for: WB, ICC, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Transferrin Receptor antibody
See all Transferrin Receptor primary antibodies -
Description
Rabbit polyclonal to Transferrin Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Dog, Pig, Orangutan
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Immunogen
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Positive control
- ICC: HeLa cells, WB: HeLa, Jurkat and U-2 OS whole cell lysates; Mouse spleen tissue lysate. Flow Cyt: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (9) |
Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).
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| ICC |
Use a concentration of 5 µg/ml.
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| Flow Cyt | (1) |
Use 1µg for 106 cells.
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| Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa). |
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ICC
Use a concentration of 5 µg/ml. |
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Flow Cyt
Use 1µg for 106 cells. |
Target
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Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
Involvement in disease
Immunodeficiency 46 -
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
Post-translational
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 7037 Human
- Entrez Gene: 22042 Mouse
- Entrez Gene: 100171944 Orangutan
- Omim: 190010 Human
- SwissProt: P02786 Human
- SwissProt: Q62351 Mouse
- SwissProt: Q5RDH6 Orangutan
- Unigene: 529618 Human
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Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Images
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Western blot - Anti-Transferrin Receptor antibody (ab84036)All lanes : Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) whole cell lysate
Lane 3 : U-2 OS (Human osteosarcoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Western blot - Anti-Transferrin Receptor antibody (ab84036)Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml + Mouse spleen tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry - Anti-Transferrin Receptor antibody (ab84036)ab84036 staining Transferrin receptor protein 1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab84036 at 5 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Flow Cytometry - Anti-Transferrin Receptor antibody (ab84036)Overlay histogram showing MCF7 cells stained with ab84036 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab84036) (1x106 in 100µl at 1 µg/ml) for 30 min at 22ºC.
The secondary antibody ab150081 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed) was used at 1/2000 for 30 min at 22ºC.
Isotype control antibody (black line) was Rabbit IgG (polyclonal) (ab171870) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (135)
ab84036 has been referenced in 135 publications.
- Furihata T et al. Cardiac-specific loss of mitoNEET expression is linked with age-related heart failure. Commun Biol 4:138 (2021). PubMed: 33514783
- Menghini S et al. Magnetospirillum magneticum as a Living Iron Chelator Induces TfR1 Upregulation and Decreases Cell Viability in Cancer Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33419059
- Shibata Y et al. Transferrin-based radiolabeled probe predicts the sensitivity of human renal cancer cell lines to ferroptosis inducer erastin. Biochem Biophys Rep 26:100957 (2021). PubMed: 33681481
- Tian X et al. Construction of a robust prognostic model for adult adrenocortical carcinoma: Results from bioinformatics and real-world data. J Cell Mol Med 25:3898-3911 (2021). PubMed: 33626208
- Jing X et al. The detrimental effect of iron on OA chondrocytes: Importance of pro-inflammatory cytokines induced iron influx and oxidative stress. J Cell Mol Med 25:5671-5680 (2021). PubMed: 33942503