Anti-Transferrin Receptor antibody (ab84036)
- Datasheet
- Specific References (38)
- Protocols
Overview
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Product nameAnti-Transferrin Receptor antibody
See all Transferrin Receptor primary antibodies -
DescriptionRabbit polyclonal to Transferrin Receptor
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-FrFlmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Dog, Pig, Orangutan
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Transferrin Receptor.
(Peptide available as ab101219.)
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Positive control
- This antibody gave a positive signal in mouse spleen tissue lysate as well as the following human whole cell lysates: HeLa; Jurkat; U20S.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Related Products
Applications
Our Abpromise guarantee covers the use of ab84036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa). | |
| IHC-P | 1/6000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-FrFl | 1/200. |
Target
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FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
Involvement in diseaseImmunodeficiency 46
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Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
Post-translational
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 7037 Human
- Entrez Gene: 22042 Mouse
- Entrez Gene: 100171944 Orangutan
- Omim: 190010 Human
- SwissProt: P02786 Human
- SwissProt: Q62351 Mouse
- SwissProt: Q5RDH6 Orangutan
- Unigene: 529618 Human
see all -
Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Images
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Western blot - Anti-Transferrin Receptor antibody (ab84036)All lanes : Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 98 kDa (why is the actual band size different from the predicted?)
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody (ab84036)Image courtesy of Carl Hobbs, King College London, U.K.ab84036 immunohistochemical staining in the rat brain. Sampled were fixed with formaldehyade and heat-mediated antigen retrieval was performed with citric acid. Tissue sections were blocked in 1% BSA for 10 minutes at 21°C before incubation with ab84036 (1/6000) for 16 hours at 21°C. A biotin conjugated goat anti-rabbit IgG secondary was used at 1/250.
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Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab84036)ICC/IF image of ab84036 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab84036 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Western blot - Anti-Transferrin Receptor antibody (ab84036)Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml + Spleen (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 100,50 kDa (why is the actual band size different from the predicted?)
Exposure time: 8 minutes
Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody (ab84036)This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhuryab84036 staining mouse liver sections by IHC-FrFl. The animal was perfused with 4% paraformaldehyde, further post fixed with 4% paraformaldehyde overnight and prepared for free floating sectioning. Staining with ab84036 at a 1/200 dilution in 0.1% TritonX with 0.1x PBS and 10% donkey was performed for 24h at 24°C. A donkey anti-rabbit Alexa568 polyclonal antibody was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab84036)Image courtesy of an anonymous Abreview.ab84036 staining Transferrin Receptor in canine kidney cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized with 0.5% saponin then incubated with ab84036 at a 1/100 dilution for 1.5 hours at 20°C. The secondary used was an Alexa-Fluor 568 conjugated donkey anti-rabbit polyclonal, used at a 1/350 dilution.
Protocols
References
This product has been referenced in:
- Connor MG et al. Yersinia pestis Targets the Host Endosome Recycling Pathway during the Biogenesis of theYersinia-Containing Vacuole To Avoid Killing by Macrophages. MBio 9:N/A (2018). Read more (PubMed: 29463656) »
- Bajbouj K et al. High-Dose Deferoxamine Treatment Disrupts Intracellular Iron Homeostasis, Reduces Growth, and Induces Apoptosis in Metastatic and Nonmetastatic Breast Cancer Cell Lines. Technol Cancer Res Treat 17:1533033818764470 (2018). Read more (PubMed: 29562821) »
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