Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598)
Key features and details
- Mouse monoclonal [B349 (DF1513)] to Transferrin Receptor
- Suitable for: IHC-Fr, ICC/IF, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Transferrin Receptor antibody [B349 (DF1513)]
See all Transferrin Receptor primary antibodies -
Description
Mouse monoclonal [B349 (DF1513)] to Transferrin Receptor -
Host species
Mouse -
Specificity
This antibody reacts with the transferrin receptor, a 180-190 kD transmembrane glycoprotein which exists as a 95 kD homodimer with interchain disulfide bond. The specificity of these antibodies, as demonstrated by immunoprecipitation, are equivalent to OKT9, B3/25 and BerT9. This antibody reacts with many proliferating cells in both normal and neoplastic tissues. It also reacts with renal tubular epithelium, islets of Langerhans, scattered cells in the anterior pituitary, hepatocytes and most tissue macrophages. -
Tested applications
Suitable for: IHC-Fr, ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Transferrin Receptor. Cell line KG1.
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Positive control
- Tonsil.
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General notes
This antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
This antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas. -
Clonality
Monoclonal -
Clone number
B349 (DF1513) -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
Target
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Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
Involvement in disease
Immunodeficiency 46 -
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
Post-translational
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 7037 Human
- Omim: 190010 Human
- SwissProt: P02786 Human
- Unigene: 529618 Human
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Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab8598 has been referenced in 6 publications.
- Shytaj IL et al. Alterations of redox and iron metabolism accompany the development of HIV latency. EMBO J 39:e102209 (2020). PubMed: 32157726
- Huang Y et al. TFRC promotes epithelial ovarian cancer cell proliferation and metastasis via up-regulation of AXIN2 expression. Am J Cancer Res 10:131-147 (2020). PubMed: 32064157
- Lehmann M et al. Nanoscale coupling of endocytic pit growth and stability. Sci Adv 5:eaax5775 (2019). PubMed: 31807703
- Perrot G et al. LRP-1--CD44, a new cell surface complex regulating tumor cell adhesion. Mol Cell Biol 32:3293-307 (2012). PubMed: 22711991
- Ortiz-Zapater E et al. Trafficking of the human transferrin receptor in plant cells: effects of tyrphostin A23 and brefeldin A. Plant J 48:757-70 (2006). PubMed: 17059402
- Ayala P et al. The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca(2+) transients in infected epithelial cells. Cell Microbiol 7:1736-48 (2005). PubMed: 16309460