Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-Transferrin Receptor antibody [B349 (DF1513)]
See all Transferrin Receptor primary antibodies -
DescriptionMouse monoclonal [B349 (DF1513)] to Transferrin Receptor
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Host speciesMouse
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SpecificityThis antibody reacts with the transferrin receptor, a 180-190 kD transmembrane glycoprotein which exists as a 95 kD homodimer with interchain disulfide bond. The specificity of these antibodies, as demonstrated by immunoprecipitation, are equivalent to OKT9, B3/25 and BerT9. This antibody reacts with many proliferating cells in both normal and neoplastic tissues. It also reacts with renal tubular epithelium, islets of Langerhans, scattered cells in the anterior pituitary, hepatocytes and most tissue macrophages.
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Tested applicationsSuitable for: WB, Flow Cyt, IHC-Fr, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Cell line KG1.
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Positive control
- Tonsil.
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General notes
This antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein G purified
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Primary antibody notesThis antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas.
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ClonalityMonoclonal
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Clone numberB349 (DF1513)
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Myelomaunknown
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab8598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100. | |
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
Involvement in diseaseImmunodeficiency 46
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Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
Post-translational
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 7037 Human
- Omim: 190010 Human
- SwissProt: P02786 Human
- Unigene: 529618 Human
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Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Images
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![Western blot - Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598)](https://www.abcam.com/ps/products/8/ab8598/Images/ab8598_1.jpg)
Western blot - Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598) at 1/100 dilution
Lane 1 : Human U2OS cell line, whole cell lysate
Lane 2 : Human 293T cell line, whole cell lysate
Lane 3 : African Green Monkey Cos7 cell line, whole cell lysate
Lane 4 : Hamster CHO cell line, whole cell lysate
Lane 5 : Rat PC12 cell line, whole cell lysate
Lane 6 : Mouse 3T3 cell line, whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-mouse antibody
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 7 minutes
Protocols
References
This product has been referenced in:
- Perrot G et al. LRP-1--CD44, a new cell surface complex regulating tumor cell adhesion. Mol Cell Biol 32:3293-307 (2012). Read more (PubMed: 22711991) »
- Ortiz-Zapater E et al. Trafficking of the human transferrin receptor in plant cells: effects of tyrphostin A23 and brefeldin A. Plant J 48:757-70 (2006). Read more (PubMed: 17059402) »
Customer reviews and Q&As
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