Anti-Transferrin Receptor antibody [DF1513] (Biotin) (ab7350)


  • Product name
    Anti-Transferrin Receptor antibody [DF1513] (Biotin)
    See all Transferrin Receptor primary antibodies
  • Description
    Mouse monoclonal [DF1513] to Transferrin Receptor (Biotin)
  • Host species
  • Conjugation
  • Specificity
    DF1513 recognises the human CD71 cell surface antigen, a 190 kD homodimeric glycoprotein.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Rhesus monkey
  • Immunogen

    KG1 acute myeloid leukemia cell line



Our Abpromise guarantee covers the use of ab7350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow cytometry: neat. Use 10 µl to label 106 cells.
    Does not work in immunohistology on frozen and paraffin-embedded sections.
  • Target

    • Function
      Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
    • Involvement in disease
      Immunodeficiency 46
    • Sequence similarities
      Belongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.
    • Post-translational
      N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
    • Cellular localization
      Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • IMD46 antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all


    This product has been referenced in:
    • Ayala P  et al. The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca(2+) transients in infected epithelial cells. Cell Microbiol 7:1736-48 (2005). Read more (PubMed: 16309460) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for your enquiry. Ab8598 and Ab7350 are the same clone, DF1513. We get these two antibodies from two different sources. Ab8598 has been tested for application in IHC-frozen sections. Unfortunately, we are not aware of any publications that fe...

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    Thank you for your enquiry. Any human cell line eg. KG1, U266, MOLT4 (i.e. anything that is proliferating well) would represent a suitable positive control for this antibody in flow cytometry.

    As we would not have tested the anti-human CD71 antibody in cell monolayers/smears we would be unable to guarantee its suitability for this procedure. However, having said that, fixing cells on slides and staining them is probably the most simple imm...

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