Recombinant
RabMAb

Recombinant Anti-Transferrin Receptor antibody [EPR4012] - Low endotoxin, Azide free (ab228995)

Overview

  • Product name

    Anti-Transferrin Receptor antibody [EPR4012] - Low endotoxin, Azide free
    See all Transferrin Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR4012] to Transferrin Receptor - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Transferrin Receptor aa 1-100. Synthetic peptide against the region close to the N terminal cytoplasmic domain of Human Transferrin Receptor (UniProt P02786).

  • Positive control

    • Human placenta, TF-1, HeLa and A549 cell lysates
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab228995 is a PBS-only buffer version of ab108985, containing no BSA or sodium azide, ideal for antibody labeling, Please refer to ab108985 for information on protocols, dilutions, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties

Applications

Our Abpromise guarantee covers the use of ab228995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
    • Involvement in disease

      Immunodeficiency 46
    • Sequence similarities

      Belongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.
    • Post-translational
      modifications

      N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
    • Cellular localization

      Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • IMD46 antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all

    Images

    • This IHC data was generated using the same anti-Transferrin Receptor antibody clone, EPR4012, in a different buffer formulation (cat# ab108985).

      ab108985 staining Transferrin Receptor in Human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000 in TBS/BSA/Azide) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    • This Flow Cyt data was generated using the same anti-Transferrin Receptor antibody clone, EPR4012, in a different buffer formulation (cat# ab108985).

      Overlay histogram showing Jurkat cells stained with ab108985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108985, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References

    ab228995 has not yet been referenced specifically in any publications.

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