Product nameAnti-Transferrin Receptor antibody [ER-MP21]
See all Transferrin Receptor primary antibodies
DescriptionRat monoclonal [ER-MP21] to Transferrin Receptor
SpecificityThis antibody reacts with Transferrin Receptor, it does not compete with transferrin binding.
Tested applicationsSuitable for: IHC-Fr, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse
Mouse CD71 Transferrin Receptor 1 (TFRC).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituents: PBS, 0.1% BSA
Concentration information loading...
Purification notesPurity: 0.2µm filtered.
Our Abpromise guarantee covers the use of ab60344 in the following tested applications.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration. PubMed: 23316765|
FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Involvement in diseaseImmunodeficiency 46
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
ab60344 has been referenced in 4 publications.
- Tan T et al. Deep Tumor-Penetrated Nanocages Improve Accessibility to Cancer Stem Cells for Photothermal-Chemotherapy of Breast Cancer Metastasis. Adv Sci (Weinh) 5:1801012 (2018). PubMed: 30581704
- Eimer WA & Vassar R Neuron loss in the 5XFAD mouse model of Alzheimer's disease correlates with intraneuronal Aß42 accumulation and Caspase-3 activation. Mol Neurodegener 8:2 (2013). ICC/IF ; Mouse . PubMed: 23316765
- Kandalepas PC et al. The Alzheimer's ß-secretase BACE1 localizes to normal presynaptic terminals and to dystrophic presynaptic terminals surrounding amyloid plaques. Acta Neuropathol 126:329-52 (2013). IHC-FrFl ; Mouse . PubMed: 23820808
- Schliemann C et al. In vivo biotinylation of the vasculature in B-cell lymphoma identifies BST-2 as a target for antibody-based therapy. Blood 115:736-44 (2010). IHC-Fr ; Mouse . PubMed: 19903902