Recombinant

Recombinant Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)

Submit a review Submit a question

Overview

  • Product name

    Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free
    See all Transferrin Receptor primary antibodies

  • Description

    Mouse monoclonal [H68.4] to Transferrin Receptor - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HeLa and K562 whole cell lysate; Human, mouse and rat placenta lysate. IHC-P: Human placenta and cervical carcinoma tissue. ICC/IF: K-562 and RAW 264.7 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab269514 is the carrier-free version of ab269513. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab269514 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

Our Abpromise guarantee covers the use of ab269514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/25.
WB 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).

Target

  • Function

    Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

  • Involvement in disease

    Immunodeficiency 46

  • Sequence similarities

    Belongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.

  • Post-translational
    modifications

    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

  • Cellular localization

    Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • IMD46 antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Images

  • Western blot - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    Western blot - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    All lanes : Anti-Transferrin Receptor antibody [H68.4] (ab269513) at 1/5000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 3 : Human placenta tissue lysate
    Lane 4 : Mouse placenta tissue lysate
    Lane 5 : Rat placenta tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 84 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    Transferrin receptor can undergo glycosylation as shown in lane 4 and 5 (PMID: 30854239).

    Blocking/Diliution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1/3-5: 114 seconds; Lane 2: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulatio containing PBS, BSA, glycerol and sodium azide (ab269513).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Transferrin Receptor with ab269513 at 1/2000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human placenta. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269513).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)

    Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling Transferrin Receptor with ab269513 at 1/2000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cervical carcinoma. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269513).

  • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K-562 cells labelling Transferrin Receptor with ab269513 at 1/25 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in K-562 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269513).

  • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling Transferrin Receptor with ab269513 at 1/25 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269513).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab269514? Please let us know so that we can cite the reference in this datasheet.

ab269514 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab269514.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com