Overview

  • Product name
    Anti-Transferrin Receptor antibody [MEM-189]
    See all Transferrin Receptor primary antibodies
  • Description
    Mouse monoclonal [MEM-189] to Transferrin Receptor
  • Host species
    Mouse
  • Specificity
    The antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.
  • Tested applications
    Suitable for: IHC-FrFl, Flow Cyt, IP, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Transferrin Receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab1086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl Use at an assay dependent concentration.
Flow Cyt Use a concentration of 2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
    • Involvement in disease
      Immunodeficiency 46
    • Sequence similarities
      Belongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.
    • Post-translational
      modifications
      N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
    • Cellular localization
      Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • IMD46 antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all

    Images

    • ab1086 staining Transferrin Receptor in Human PC-3 prostate cancer cell line by Flow Cytometry. The sample was incubated with the primary antibody (0.3µg/ml in PBS + 2% FBS) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG (1/500) was used as the secondary antibody.
      Gating Strategy: Cells gated for live and singlets.

      See Abreview

    References

    This product has been referenced in:
    • Wang Z  et al. Pseudolaric acid B triggers ferroptosis in glioma cells via activation of Nox4 and inhibition of xCT. Cancer Lett 428:21-33 (2018). Read more (PubMed: 29702192) »
    • Sui M  et al. Magnesium isoglycyrrhizinate ameliorates liver fibrosis and hepatic stellate cell activation by regulating ferroptosis signaling pathway. Biomed Pharmacother 106:125-133 (2018). Read more (PubMed: 29957462) »
    See all 15 Publications for this product

    Customer reviews and Q&As

    1-10 of 15 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (Kidney)
    Permeabilization
    Yes - 0.2% triton x 100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 27 2017

    Application
    Flow Cytometry
    Fixation
    None
    Permeabilization
    No
    Sample
    Human Cell (PC-3 Prostrate cancer cell line)
    Specification
    PC-3 Prostrate cancer cell line
    Gating Strategy
    Cells gated for live and singlets
    Preparation
    Cell harvesting/tissue preparation method: Cells detached with PBS-based cell dissociation buffer, collected by centrifugation and re-suspended in PBS + 2% FBS
    Sample buffer: PBS + 2% FBS

    Abcam user community

    Verified customer

    Submitted Nov 05 2013

    Answer

    Thank you for contacting us.

    According to our datasheet, this antibody only works under non-reducing conditions, I am afraid: it may be that the antibody cannot detect the reduced protein.Otherwise, it should workwith the use of your usual protocol as we do not have any other restrictions on the datasheet. As a side note, we usually use 3%BSA as a blocking agent as we find that it gives a significant stronger signal with monoclonal antibodies.

    According to SwissProt, this protein has a molecular weight of 85 kDa when unmodified. However, as it has many glycosylation sides, I would expect it to run higher; the glycosylation status will influence the weight.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thank you for contacting us.

    Unfortunately the epitope of ab1086 has not been mapped. We only know that this antibody recognise an extracellular part of Transferrin Receptor.

    I am sorry for not being able to provide any useful information this time. Please do not hesitate to contact us if you need any more advice or information in other aspect.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Answer

    Thank you for contacting us.

    Yes, the extra blocking step is always required to avoid the mouse back ground. We have recommendations that can be found by clicking the following link;

    https://www.abcam.com/index.html?pageconfig=resource&rid=11465

    We have kit which is perfect for mouse on mouse staining; please have a look at the datasheet

    https://www.abcam.com/Mouse-on-Mouse-MOM-Polymer-IHC-Kit-ab127055.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question
    Answer

    Thank you for your enquiry.

    We have not tested these products (ab1086, ab84036, ab9179, ab8598) for cross reactivity with Trf2. However, based on immunogen sequence (ClustalW analysis, Score 23.0), we do not expect to see any cross reactivity whatsoever.

    I hope this information will help settle any concerns that you may have.

    Read More

    Answer

    Thank you for bringing that to my attention.
    I did some additional research into CD71. CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. Ab1086 used under non-reducing conditions should recognize the homodimer at greater than 170kDa after post translation modifications. Did you see any bands in that range?
    If used in reducing conditions it seems that the product should pick up the band around 85kDa (before PTM). The datasheet is not very clear about this. When repeating your experiments I would recommend looking for the larger weight. It may also be useful to try reducing conditions as well.
    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    Answer

    Thank you for your response.

    I am very sorry to say this but we cannot accept returned items that have been frozen. This is because we need to limit any freeze/thaw cycles that the product might experience. I am sorry about the inconvenience that this might cause.

    May I suggest however that you repeat your experiment using one of these vials? I have had the opportunity to review your protocol, which looks fine. If your results are the same (a band at less than 1/2 the expected weight) then we might conclude that the issue is with the product. In which case I might refund or replace all the products without need for return.

    Please let me know if this is acceptable for you and if you have any questions.

    Read More

    Answer

    I am sorry that you have been experiencing problems with one of our products and wish to thank you for bringing this to our attention. As this order was placed and delivered within the last seven day I may issue a return for you. Since this is ultimately a problem with one of our product I am waiving the standard 20% restocking fee for you.

    Before I may process this return however I will need a little information:

    Your order was for three vials of ab1086. Have any of these been opened?

    How have these products been stored since delivery?

    I would also like to discuss the original vial of ab1086 with you and perhaps issue a free of charge replacement or refund for that product. In order to do this I will need some information about your experiments with this product. Could you please send me the protocol used?

    Would you also be able to send me the order number for the original vial?

    Please let me know if you have any questions.

    Read More

    1-10 of 15 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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