Key features and details
- Mouse monoclonal [MEM-189] to Transferrin Receptor
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Transferrin Receptor antibody [MEM-189]
See all Transferrin Receptor primary antibodies
DescriptionMouse monoclonal [MEM-189] to Transferrin Receptor
SpecificityThe antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human Transferrin Receptor. KG1 human acute myelogenous leukaemia cell line
- Flow Cyt: K562 cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.0
Preservative: 0.097% Sodium azide
Constituent: Tris buffer
Concentration information loading...
PurityTissue culture supernatant
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml.
Use a concentration of 2 µg/ml.
FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Involvement in diseaseImmunodeficiency 46
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
ab1086 has been referenced in 26 publications.
- Shan X et al. The Protective Effect of Cyanidin-3-Glucoside on Myocardial Ischemia-Reperfusion Injury through Ferroptosis. Oxid Med Cell Longev 2021:8880141 (2021). PubMed: 33628391
- Zeng X et al. Benefits of Iron Chelators in the Treatment of Parkinson's Disease. Neurochem Res 46:1239-1251 (2021). PubMed: 33646533
- Song Z et al. Ruscogenin induces ferroptosis in pancreatic cancer cells. Oncol Rep 43:516-524 (2020). PubMed: 31894321
- Di Marco A et al. Establishment of an in Vitro Human Blood-Brain Barrier Model Derived from Induced Pluripotent Stem Cells and Comparison to a Porcine Cell-Based System. Cells 9:N/A (2020). PubMed: 32316221
- Zhou Y The Protective Effects of Cryptochlorogenic Acid on ß-Cells Function in Diabetes in vivo and vitro via Inhibition of Ferroptosis. Diabetes Metab Syndr Obes 13:1921-1931 (2020). PubMed: 32606852