Anti-Transferrin Receptor antibody [MEM-75] (Allophycocyanin/Cy7 ®) (ab233254)

Overview

  • Product name

    Anti-Transferrin Receptor antibody [MEM-75] (Allophycocyanin/Cy7 ®)
    See all Transferrin Receptor primary antibodies
  • Description

    Mouse monoclonal [MEM-75] to Transferrin Receptor (Allophycocyanin/Cy7 ®)
  • Host species

    Mouse
  • Conjugation

    Allophycocyanin/Cy7 ®. Ex: 650nm, Em: 774nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Transferrin Receptor. NALM-6 human pre-B cell line.

  • Positive control

    • Flow Cytometry: K562 cells and human lymphocytes.
  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

Applications

Our Abpromise guarantee covers the use of ab233254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 4µl for 106 cells.

Target

  • Function

    Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in disease

    Immunodeficiency 46
  • Sequence similarities

    Belongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications

    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localization

    Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • IMD46 antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Images

  • Surface staining of Transferrin Receptor in K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells (red) and human lymphocytes (negative, grey) using ab233254.

References

ab233254 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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