Key features and details
- Mouse monoclonal [MEM-75] to Transferrin Receptor (APC/Cy7®)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC/Cy7®. Ex: 650nm, Em: 774nm
- Isotype: IgG1
Product nameAnti-Transferrin Receptor antibody [MEM-75] (APC/Cy7®)
See all Transferrin Receptor primary antibodies
DescriptionMouse monoclonal [MEM-75] to Transferrin Receptor (APC/Cy7®)
ConjugationAPC/Cy7®. Ex: 650nm, Em: 774nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human Transferrin Receptor. NALM-6 human pre-B cell line.
- Flow Cytometry: K562 cells and human lymphocytes.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferPreservative: 0.0975% Sodium azide
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- Anti-Transferrin Receptor antibody [MEM-75] (PE) (ab18242)
- Anti-Transferrin Receptor antibody [MEM-75] (FITC) (ab239251)
- Anti-Transferrin Receptor antibody [MEM-75] (PerCP/Cy5.5®) (ab239340)
- Anti-CD43 antibody [MEM-59] (Alexa Fluor® 700) (ab270666)
- Anti-Transferrin Receptor antibody [MEM-75] (Alexa Fluor® 700) (ab270667)
- Anti-Transferrin Receptor antibody [MEM-75] (Biotin) (ab28116)
- Anti-Transferrin Receptor antibody [MEM-75], prediluted (APC) (ab64672)
- Anti-Transferrin Receptor antibody [MEM-75] (ab9179)
Our Abpromise guarantee covers the use of ab233254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 4µl for 106 cells.|
FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Involvement in diseaseImmunodeficiency 46
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233254 has not yet been referenced specifically in any publications.