Product nameAnti-Transferrin Receptor antibody [MEM-75] (Biotin)
See all Transferrin Receptor primary antibodies
DescriptionMouse monoclonal [MEM-75] to Transferrin Receptor (Biotin)
Specificityab28116 recognises CD71 antigen (transferrin receptor).
Tested applicationsSuitable for: Flow Cyt, IP, WBmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human Transferrin Receptor.
ab28116 does not block binding of transferrin to the receptor.
The purified antibody is conjugated with Biotin-LC-NHS.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityProtein A purified
Primary antibody notesab28116 does not block binding of transferrin to the receptor.
- Anti-Transferrin Receptor antibody [MEM-75] (Phycoerythrin) (ab18242)
- Anti-Transferrin Receptor antibody [MEM-75] (Allophycocyanin/Cy7 ®) (ab233254)
- Anti-Transferrin Receptor antibody [MEM-75] (FITC) (ab239251)
- Anti-Transferrin Receptor antibody [MEM-75], prediluted (Allophycocyanin) (ab64672)
- Anti-Transferrin Receptor antibody [MEM-75] (ab9179)
Our Abpromise guarantee covers the use of ab28116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Involvement in diseaseImmunodeficiency 46
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
ab28116 has not yet been referenced specifically in any publications.