Key features and details
- Mouse monoclonal [MEM-75] to Transferrin Receptor, prediluted (APC)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Product nameAnti-Transferrin Receptor antibody [MEM-75], prediluted (APC)
See all Transferrin Receptor primary antibodies
DescriptionMouse monoclonal [MEM-75] to Transferrin Receptor, prediluted (APC)
ConjugationAPC. Ex: 645nm, Em: 660nm
Specificityab64672 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. ab64672 does not block binding of transferrin to the receptor.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
NALM-6 human pre-B cell line
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
Purification notesThe purified antibody (>95% by SDS-PAGE) is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
- Anti-Transferrin Receptor antibody [MEM-75] (PE) (ab18242)
- Anti-Transferrin Receptor antibody [MEM-75] (APC/Cy7®) (ab233254)
- Anti-Transferrin Receptor antibody [MEM-75] (FITC) (ab239251)
- Anti-Transferrin Receptor antibody [MEM-75] (PerCP/Cy5.5®) (ab239340)
- Anti-CD43 antibody [MEM-59] (Alexa Fluor® 700) (ab270666)
- Anti-Transferrin Receptor antibody [MEM-75] (Alexa Fluor® 700) (ab270667)
- Anti-Transferrin Receptor antibody [MEM-75] (Biotin) (ab28116)
- Anti-Transferrin Receptor antibody [MEM-75] (ab9179)
Our Abpromise guarantee covers the use of ab64672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Involvement in diseaseImmunodeficiency 46
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
ab64672 has not yet been referenced specifically in any publications.