Anti-Transferrin Receptor antibody [TFRC/1818] - Extracellular domain (ab218326)

Overview

  • Product name

    Anti-Transferrin Receptor antibody [TFRC/1818] - Extracellular domain
    See all Transferrin Receptor primary antibodies
  • Description

    Mouse monoclonal [TFRC/1818] to Transferrin Receptor - Extracellular domain
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Transferrin Receptor aa 94-212 (extracellular). The exact sequence is proprietary.
    Database link: P02786

  • Positive control

    • Jurkat, HeLa, MCF-7 or K562 cells. Human placenta, bone marrow and breast carcinoma tissues.
  • General notes

    Anti-Transferrin Receptor antibody [TFRC/1818] ab218326 is directed against Extracellular domain.

Properties

Applications

Our Abpromise guarantee covers the use of ab218326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

Target

  • Function

    Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in disease

    Immunodeficiency 46
  • Sequence similarities

    Belongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications

    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localization

    Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • IMD46 antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling Transferrin Receptor with ab218326 at 2 µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bone marrow labeling Transferrin Receptor with ab218326.

References

ab218326 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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