Product nameAnti-Transferrin Receptor antibody [YTA 74.4]
See all Transferrin Receptor primary antibodies
DescriptionRat monoclonal [YTA 74.4] to Transferrin Receptor
SpecificityThis antibody detects mouse CD71 antigen, the transferrin receptor (TFR). Expressed on all dividing cells and can distinguish resting and activated T-cells. Immunoprecipitates a disulphide linked homodimer of 90kD by SDS PAGE. The antibody inhibits cell proliferation and the mixed lymphocyte response in vitro. Blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies.
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IP, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat
Tissue/ cell preparation (Mouse). Mouse Concanavilin A activated spleen cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein G purified
Clone numberYTA 74.4
Our Abpromise guarantee covers the use of ab22391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20457837|
|Flow Cyt||1/50 - 1/100.
Use 10µl of the suggested working dilution to label 1 x 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Involvement in diseaseImmunodeficiency 46
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
This product has been referenced in:
- Xia X et al. Porous silicon microparticle potentiates anti-tumor immunity by enhancing cross-presentation and inducing type I interferon response. Cell Rep 11:957-966 (2015). Read more (PubMed: 25937283) »
- Mukai Y et al. Induction of endoplasmic reticulum-endosome fusion for antigen cross-presentation induced by poly (?-glutamic acid) nanoparticles. J Immunol 187:6249-55 (2011). IHC (PFA fixed) ; Mouse . Read more (PubMed: 22095716) »