Key features and details
- Rabbit polyclonal to Transferrin Receptor (phospho S24)
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Transferrin Receptor (phospho S24) antibody
See all Transferrin Receptor primary antibodies
DescriptionRabbit polyclonal to Transferrin Receptor (phospho S24)
SpecificityDetects endogenous levels of Transferrin Receptor only when phosphorylated at serine 24.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phosphopeptide derived from human Transferrin Receptor around the phosphorylation site of serine 24 (R-F-SP-L-A).
- Human brain tissue 293 cells, treated with PMA
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab61021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Involvement in diseaseImmunodeficiency 46
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab61021 at a dilution of 1/50.
All lanes : Anti-Transferrin Receptor (phospho S24) antibody (ab61021)
Lane 1 : extracts from 293 cells, treated
with PMA (125ng/ml, 30mins)
Lane 2 : extracts from 293 cells, treated
with PMA (125ng/ml, 30mins) with Immunizing phospho-peptide
Predicted band size: 85 kDa
Observed band size: 85 kDa
ab61021 has been referenced in 1 publication.
- Lee J et al. Investigation of Transcriptional Gene Profiling in Normal Murine Hair Follicular Substructures Using Next-Generation Sequencing to Provide Potential Insights Into Skin Disease. Cell Transplant 25:377-99 (2016). PubMed: 25995029