Anti-Transglutaminase 2 antibody (ab421)

ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immnohistochemical analysis of murine intestine tissue treated with poly(I:C), staining Transglutaminase 2 with ab421.

Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 for 10 min at RT, then blocked in 0.3% Triton X-100 and 5% goat serum in PBS. Samples were incubated with primary antibody (1/250) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.