The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 5 - 10 µg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
IP: Use at 10µg/mg of lysate.
WB: Use at a concentration of 5 - 10 µg/ml for 2hrs at RT. Predicted molecular weight: 77 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
Belongs to the transglutaminase superfamily. Transglutaminase family.