Anti-Transglutaminase 2 antibody - C-terminal (ab183539)
Key features and details
- Goat polyclonal to Transglutaminase 2 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Transglutaminase 2 antibody - C-terminal
See all Transglutaminase 2 primary antibodies -
Description
Goat polyclonal to Transglutaminase 2 - C-terminal -
Host species
Goat -
Specificity
ab183539 is expected to recognise isoform a (NP_004604.2) only. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human Transglutaminase 2 aa 650 to the C-terminus (C terminal) (Cysteine residue). (NP_004604.2).
Database link: P21980 -
Positive control
- Human Pancreas cancer lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab183539 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 77 kDa).
A 1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 77 kDa). A 1 hour primary incubation is recommended for this product. |
Target
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Function
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. -
Sequence similarities
Belongs to the transglutaminase superfamily. Transglutaminase family. - Information by UniProt
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Database links
- Entrez Gene: 281528 Cow
- Entrez Gene: 7052 Human
- Entrez Gene: 21817 Mouse
- Entrez Gene: 56083 Rat
- Omim: 190196 Human
- SwissProt: P51176 Cow
- SwissProt: P21980 Human
- SwissProt: P21981 Mouse
see all -
Alternative names
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 2.3.2.13 antibody
see all
Images
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Anti-Transglutaminase 2 antibody - C-terminal (ab183539) at 0.05 µg/ml + Human Pancreas cancer lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (0)
ab183539 has not yet been referenced specifically in any publications.