Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)
![Western blot - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)](https://www.abcam.com/ps/products/2/ab2386/Images/ab2386-367611-anti-transglutaminase-2-antibody-cub-7402-western-blot-knockout-hela-huvec-og-a549-wt-human.jpg "Western blot - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)")
Key features and details
- Mouse monoclonal [CUB 7402] to Transglutaminase 2
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rabbit, Guinea pig, Cat, Dog, Human
- Isotype: IgG1
Overview
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Product name
Anti-Transglutaminase 2 antibody [CUB 7402]
See all Transglutaminase 2 primary antibodies -
Description
Mouse monoclonal [CUB 7402] to Transglutaminase 2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rabbit, Guinea pig, Cat, Dog, Human -
Immunogen
Full length native protein (purified) corresponding to Transglutaminase 2. Purified from liver.
Database link: P08587 -
Epitope
Epitope mapping for this antibody is amino acid 447-478 -
Positive control
- IHC-P: Human breast carcinoma tissue. WB: A549 whole cell lysate; HeLa whole cell lysate; Huvec whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
CUB 7402 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
1/50 - 1/75. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
|
| ICC/IF | (2) |
Use at an assay dependent concentration. PubMed: 19398782
|
| Flow Cyt | (1) |
1/10.
Methanol fixed cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IHC-P
1/50 - 1/75. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 19398782 |
|
Flow Cyt
1/10. Methanol fixed cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. -
Sequence similarities
Belongs to the transglutaminase superfamily. Transglutaminase family. - Information by UniProt
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Database links
- Entrez Gene: 7052 Human
- Entrez Gene: 21817 Mouse
- Entrez Gene: 100352148 Rabbit
- Omim: 190196 Human
- SwissProt: P21980 Human
- SwissProt: P21981 Mouse
- Unigene: 517033 Human
- Unigene: 330731 Mouse
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Alternative names
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 2.3.2.13 antibody
see all
Images
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Western blot - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)All lanes : Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386) at 1 µg/ml
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : TGM2 knockout A549 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Huvec whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 77 kDa
Observed band size: 77 kDaLanes 1 - 4: Merged signal (red and green). Green - ab2386 observed at 77 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab2386 was shown to recognize in wild-type A549 cells as signal was lost at the expected MW in TGM2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab2386 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)](https://www.abcam.com/ps/products/2/ab2386/Images/ab2386-391638-anti-transglutaminase-2-antibody-cub-7402-immunohistochemistry-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)Immunohistochemical analysis of breast carcinoma tissue labeling Transglutaminase 2 with ab2386. Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 30 minutes at 22°C.
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![Flow Cytometry - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)](https://www.abcam.com/ps/products/2/ab2386/Images/ab2386-5751-anti-transglutaminase-2-antibody-cub-7402-flow-cytometry.jpg)
Flow Cytometry - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)Overlay histogram showing HCT116 cells stained with ab2386 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2386, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HCT116 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)](https://www.abcam.com/ps/products/2/ab2386/Images/ab2386-275686-anti-transglutaminase-2-antibody-cub-7402-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) rat lung tissue labeling Transglutaminase 2 with ab2386. Tissue was fixed with formaldehyde and blocked with 1% casein in PBS for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6. Samples were incubated with primary antibody (1/200 in TBS + BSA) for 20 minutes. ab98782 was used as the secondary antibody at a dilution of 1/400.
Protocols
Datasheets and documents
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Datasheet download
References (27)
ab2386 has been referenced in 27 publications.
- Sun W et al. The NEAT1_2/miR-491 Axis Modulates Papillary Thyroid Cancer Invasion and Metastasis Through TGM2/NF?b/FN1 Signaling. Front Oncol 11:610547 (2021). PubMed: 33738254
- Aonuma E et al. Nickel ions attenuate autophagy flux and induce transglutaminase 2 (TG2) mediated post-translational modification of SQSTM1/p62. Biochem Biophys Res Commun 542:17-23 (2021). PubMed: 33482469
- Potilinski MC et al. Elucidating the mechanism of action of alpha-1-antitrypsin using retinal pigment epithelium cells exposed to high glucose. Potential use in diabetic retinopathy. PLoS One 15:e0228895 (2020). PubMed: 32032388
- Bordeleau F et al. Tissue transglutaminase 2 regulates tumor cell tensional homeostasis by increasing contractility. J Cell Sci 133:N/A (2020). PubMed: 31822629
- Deng Z et al. Characterization of articular cartilage homeostasis and the mechanism of superior cartilage regeneration of MRL/MpJ mice. FASEB J N/A:fj201802132RR (2019). PubMed: 31042406
