Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)
- Datasheet
- References (15)
- Protocols
Overview
-
Product nameAnti-Transglutaminase 2 antibody [CUB 7402]
See all Transglutaminase 2 primary antibodies -
DescriptionMouse monoclonal [CUB 7402] to Transglutaminase 2
-
Host speciesMouse
-
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
-
Species reactivityReacts with: Mouse, Rabbit, Guinea pig, Cat, Dog, Human
-
Immunogen
Purified guinea pig liver Transglutaminase 2.
-
EpitopeEpitope mapping for this antibody is amino acid 447-478
-
Positive control
- IHC-P: Human breast carcinoma tissue.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
PurityIgG fraction
-
ClonalityMonoclonal
-
Clone numberCUB 7402
-
Myelomaunknown
-
IsotypeIgG1
-
Light chain typeunknown
-
Research areas
Associated products
-
Compatible Secondaries
-
Immunohistochemistry kits
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab2386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/75. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. PubMed: 18397883 | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 19398782 | |
| Flow Cyt | 1/10. Methanol fixed cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
FunctionCatalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
-
Sequence similaritiesBelongs to the transglutaminase superfamily. Transglutaminase family.
- Information by UniProt
-
Database links
- Entrez Gene: 7052 Human
- Entrez Gene: 21817 Mouse
- Entrez Gene: 100352148 Rabbit
- Omim: 190196 Human
- SwissProt: P21980 Human
- SwissProt: P21981 Mouse
- Unigene: 517033 Human
- Unigene: 330731 Mouse
-
Alternative names
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 2.3.2.13 antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)](https://www.abcam.com/ps/products/2/ab2386/Images/ab2386-260303-transglutaminase.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)ab2386 staining breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 30 minutes at 22°C.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)](https://www.abcam.com/ps/products/2/ab2386/Images/Transglutaminase-2-Primary-antibodies-ab2386-6.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)This image is a courtesy of Doriana Celluraab2386 staining Transglutaminase 2 in human SW480 colorectal carcinoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in 1X PBS/ 1% BSA for 7 min at RT and permeabilized with 0.1% Triton-X100 in PBS for 5 min at RT. Samples were incubated with primary antibody (1/100: in 1X PBS / 1% BSA) for 20 minutes at 20°C. An FITC-conjugated rat polyclonal to mouse IgG was used at dilution at 1/300 as secondary antibody. Slides counterstained with DAPI, and mounted with commercially available Prolong Slow-fade mounting medium.
-
![Flow Cytometry - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)](https://www.abcam.com/ps/products/2/ab2386/Images/Transglutaminase-2-Primary-antibodies-ab2386-7.jpg)
Flow Cytometry - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)Overlay histogram showing HCT116 cells stained with ab2386 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2386, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HCT116 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)](https://www.abcam.com/ps/products/2/ab2386/Images/ab2386-275686-ab2386ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)This image is courtesy of an anonymous Abreviewab2386 staining Transglutaminase 2 in rat lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% casein in PBS for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6. Samples were incubated with primary antibody (1/200 in TBS + BSA) for 20 minutes. ab98782 was used as the secondary antibody at a dilution of 1/400.
Datasheets and documents
Protocols
References
This product has been referenced in:
- Ruvolo PP et al. Role of MSC-derived galectin 3 in the AML microenvironment. Biochim Biophys Acta 1865:959-969 (2018). Read more (PubMed: 29655803) »
- Lei Z et al. Novel peptide GX1 inhibits angiogenesis by specifically binding to transglutaminase-2 in the tumorous endothelial cells of gastric cancer. Cell Death Dis 9:579 (2018). Read more (PubMed: 29785022) »
