Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Transglutaminase 2 antibody [EP2957] (ab109200)

Overview

  • Product name

    Anti-Transglutaminase 2 antibody [EP2957]
    See all Transglutaminase 2 primary antibodies
  • Description

    Rabbit monoclonal [EP2957] to Transglutaminase 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Transglutaminase 2 aa 550-650. The exact sequence is proprietary.

  • Positive control

    • U87-MG, A549, HeLa and HUVEC cell lysates; Human kidney tissue
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 77 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
  • Sequence similarities

    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALPHA SUBUNIT antibody
    • C polypeptide antibody
    • EC 2.3.2.13 antibody
    • epididymis secretory protein Li 45 antibody
    • G alpha h antibody
    • G[a]h antibody
    • Gh CLASS G ALPHA h antibody
    • GNAH antibody
    • GNAH G PROTEIN antibody
    • H POLYPEPTIDE antibody
    • HEL-S-45 antibody
    • Protein glutamine gamma glutamyltransferase 2 antibody
    • Protein-glutamine gamma-glutamyltransferase 2 antibody
    • TG 2 antibody
    • TG(C) antibody
    • TG2 antibody
    • TGase C antibody
    • TGase H antibody
    • TGase-2 antibody
    • TgaseII antibody
    • TGC antibody
    • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
    • TGM2 antibody
    • TGM2_HUMAN antibody
    • Tissue transglutaminase antibody
    • Transglutaminase 2 antibody
    • Transglutaminase 2 C polypeptide antibody
    • Transglutaminase C antibody
    • Transglutaminase H antibody
    • Transglutaminase-2 antibody
    • tTG antibody
    • tTGas antibody
    see all

Images

  • All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/1000 dilution

    Lane 1 : Wild-type A549 whole cell lysate
    Lane 2 : TGM2 knockout A549 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HUVEC whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 77 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab109200 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109200 was shown to recognize TGM2 in wild-type A549 cells as signal was lost at the expected MW in TGM2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab109200 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/10000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 77 kDa

  • ab109200, at 1/100 dilution, staining Transglutaminase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ab109200 staining Transglutaminase 2 in mouse kidney and heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween-20 and blocked with 5% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in PBS-T + 5% BSA) for 12 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Shinde AV  et al. Pharmacologic inhibition of the enzymatic effects of tissue transglutaminase reduces cardiac fibrosis and attenuates cardiomyocyte hypertrophy following pressure overload. J Mol Cell Cardiol 117:36-48 (2018). Read more (PubMed: 29481819) »
  • Lei Z  et al. Novel peptide GX1 inhibits angiogenesis by specifically binding to transglutaminase-2 in the tumorous endothelial cells of gastric cancer. Cell Death Dis 9:579 (2018). Read more (PubMed: 29785022) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Mouse Tissue sections (Kidney, Heart)
Specification
Kidney, Heart
Permeabilization
Yes - 0.1% Tween-20
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 02 2014

Question
Answer

Thank you for your enquiry.

I can confirm that ab109200 Transglutaminase 2 antibody [EP2957] has the following alignments to the isoforms of the protein:

Isoform 1 100%
isoform 2 and 3 26%

Therefore, the antibody is not likely to detect isoform 2 and 3.

Please note this is based on alignment only and I am sorry the crossreactivity has not specifically been tested in the laboratory.

I hope this will be helpful. If you have nay further questions, please do not hesitate to contact us.

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