Product nameAnti-Transglutaminase 2 antibody [EP2957]
See all Transglutaminase 2 primary antibodies
DescriptionRabbit monoclonal [EP2957] to Transglutaminase 2
Tested applicationsSuitable for: WB, IP, IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Transglutaminase 2 aa 550-650. The exact sequence is proprietary.
- U87-MG, A549, HeLa and HUVEC cell lysates; Human kidney tissue
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab109200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 77 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionCatalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
Sequence similaritiesBelongs to the transglutaminase superfamily. Transglutaminase family.
- Information by UniProt
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 184.108.40.206 antibody
All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/1000 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : TGM2 knockout A549 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 77 kDa
Lanes 1 - 4: Merged signal (red and green). Green - ab109200 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109200 was shown to recognize TGM2 in wild-type A549 cells as signal was lost at the expected MW in TGM2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab109200 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/10000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 77 kDa
ab109200, at 1/100 dilution, staining Transglutaminase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab109200 staining Transglutaminase 2 in mouse kidney and heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween-20 and blocked with 5% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in PBS-T + 5% BSA) for 12 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody.
This product has been referenced in:
- Shinde AV et al. Pharmacologic inhibition of the enzymatic effects of tissue transglutaminase reduces cardiac fibrosis and attenuates cardiomyocyte hypertrophy following pressure overload. J Mol Cell Cardiol 117:36-48 (2018). Read more (PubMed: 29481819) »
- Lei Z et al. Novel peptide GX1 inhibits angiogenesis by specifically binding to transglutaminase-2 in the tumorous endothelial cells of gastric cancer. Cell Death Dis 9:579 (2018). Read more (PubMed: 29785022) »