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Cell Biology Apoptosis Intracellular Associated Proteins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Transglutaminase 2 antibody [EPR2956] - BSA and Azide free (ab247770)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Transglutaminase 2 antibody [EPR2956] - BSA and Azide free (ab247770)
  • Anti-Transglutaminase 2 antibody [EPR2956] - BSA and Azide free (ab247770)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2956] to Transglutaminase 2 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
Recombinant Human Transglutaminase 2 protein (ab73764)

View more associated products

Overview

  • Product name

    Anti-Transglutaminase 2 antibody [EPR2956] - BSA and Azide free
    See all Transglutaminase 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2956] to Transglutaminase 2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Transglutaminase 2 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • WB: A549 cell lysate.
  • General notes

    Ab247770 is the carrier-free version of ab109121. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247770 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2956
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-Transglutaminase 2 antibody [EPR2956] (ab109121)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • KO cell lines

    • Human TGM2 (Transglutaminase 2) knockout A549 cell line (ab267110)
  • KO cell lysates

    • Human TGM2 (Transglutaminase 2) knockout A549 cell lysate (ab257087)
  • Recombinant Protein

    • Recombinant Human Transglutaminase 2 protein (ab73764)

Applications

Our Abpromise guarantee covers the use of ab247770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 77 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
    • Sequence similarities

      Belongs to the transglutaminase superfamily. Transglutaminase family.
    • Target information above from: UniProt accession P21980 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7052 Human
      • Entrez Gene: 21817 Mouse
      • Entrez Gene: 56083 Rat
      • Omim: 190196 Human
      • SwissProt: P21980 Human
      • SwissProt: P21981 Mouse
      • Unigene: 517033 Human
      • Unigene: 330731 Mouse
      • Alternative names

        • ALPHA SUBUNIT antibody
        • C polypeptide antibody
        • EC 2.3.2.13 antibody
        • epididymis secretory protein Li 45 antibody
        • G alpha h antibody
        • G[a]h antibody
        • Gh CLASS G ALPHA h antibody
        • GNAH antibody
        • GNAH G PROTEIN antibody
        • H POLYPEPTIDE antibody
        • HEL-S-45 antibody
        • Protein glutamine gamma glutamyltransferase 2 antibody
        • Protein-glutamine gamma-glutamyltransferase 2 antibody
        • TG 2 antibody
        • TG(C) antibody
        • TG2 antibody
        • TGase C antibody
        • TGase H antibody
        • TGase-2 antibody
        • TgaseII antibody
        • TGC antibody
        • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
        • TGM2 antibody
        • TGM2_HUMAN antibody
        • Tissue transglutaminase antibody
        • Transglutaminase 2 antibody
        • Transglutaminase 2 C polypeptide antibody
        • Transglutaminase C antibody
        • Transglutaminase H antibody
        • Transglutaminase-2 antibody
        • tTG antibody
        • tTGas antibody
        see all

      Images

      • Western blot - Anti-Transglutaminase 2 antibody [EPR2956] - BSA and Azide free (ab247770)
        Western blot - Anti-Transglutaminase 2 antibody [EPR2956] - BSA and Azide free (ab247770)
        All lanes : Anti-Transglutaminase 2 antibody [EPR2956] (ab109121) at 1/1000 dilution

        Lane 1 : Wild-type A549 cell lysate
        Lane 2 : TGM2 knockout A549 cell lysate

        Lysates/proteins at 20 µg per lane.

        Performed under reducing conditions.

        Predicted band size: 77 kDa
        Observed band size: 77 kDa



        This data was developed using the same antibody clone in a different buffer formulation (ab109121).

        Lanes 1-2: Merged signal (red and green). Green - ab109121 observed at 77 kDa. Red - loading control ab8245 observed at 37 kDa.

         ab109121 Anti-Transglutaminase 2 antibody [EPR2956] was shown to specifically react with Transglutaminase 2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267110 (knockout cell lysate ab257087) was used. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. ab109121 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

         

         

      • Anti-Transglutaminase 2 antibody [EPR2956] - BSA and Azide free (ab247770)
        Anti-Transglutaminase 2 antibody [EPR2956] - BSA and Azide free (ab247770)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab247770? Please let us know so that we can cite the reference in this datasheet.

      ab247770 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab247770.
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