Product nameAnti-Transketolase antibody [7H1AA1]
See all Transketolase primary antibodies
DescriptionMouse monoclonal [7H1AA1] to Transketolase
SpecificityShotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
Tested applicationsSuitable for: IHC-P, ICC/IF, IP, WB, In-Cell ELISA, Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus. This information is considered to be commercially sensitive.
- HeLa and HepG2 whole cell lysate (ab7900); HL60 and HeLa cells. Human normal colon FFPE tissue.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline
Concentration information loading...
Purification notesab112997 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%).
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab112997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.|
|In-Cell ELISA||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Sequence similaritiesBelongs to the transketolase family.
- Information by UniProt
- EC 188.8.131.52 antibody
- FLJ34765 antibody
- TK antibody
All lanes : Anti-Transketolase antibody [7H1AA1] (ab112997) at 1 µg/ml
Lane 1 : HepG2 Cell Lysate
Lane 2 : HeLa Cell Lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 68 kDa
ab112997 at 1ug/ml staining Transketolase in HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).
ab112997 at 1ug/ml staining Transketolase in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Nuclei were stained with DAPI (blue). Non specific fluorescence was reduced using a signal enhancer.
IHC image of Transketolase staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112997, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Burrai GP et al. A first immunohistochemistry study of transketolase and transketolase-like 1 expression in canine hyperplastic and neoplastic mammary lesions. BMC Vet Res 13:38 (2017). Read more (PubMed: 28143530) »
- Yang H et al. MicroRNA-497 regulates cisplatin chemosensitivity of cervical cancer by targeting transketolase. Am J Cancer Res 6:2690-2699 (2016). WB, IHC-P . Read more (PubMed: 27904781) »