Overview

  • Product name

    Anti-Transketolase antibody
    See all Transketolase primary antibodies
  • Description

    Rabbit polyclonal to Transketolase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment (His-T7-tag) corresponding to Rat Transketolase aa 1-286. Expressed in E.coli. N-terminal tags.
    Sequence:

    MDPVRQIQCDREGKRFPFLSAPPASTSSPDRAMEGYHKPDQQKLQALKDT ANRLRISSIQATTAAGSGHPTSCCSAAEIMAVLFFHTMRYKALDPRNPHN DRFVLSKGHAAPILYAVWAEAGFLPEAELLNLRKISSDLDGHPVPKQAFT DVATGSLGQGLGAACGMAYTGKYFDKASYRVYCMLGDGEVSEGSVWEAMA FAGIYKLDNLVAIFDINRLGQSDPAPLQHQVDVYQKRCEAFGWHAIIVDG HSVEELCKAFGQAKHQPTAIIAKTFKGRGITGIEDK


    Database link: G3V826

  • Positive control

    • IHC-P: Rat colon, liver and spinal cord tissues. WB: HeLa cell lysate; Recombinant rat Transketolase protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab232819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 71 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

Images

  • Anti-Transketolase antibody (ab232819) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Secondary
    HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml

    Predicted band size: 71 kDa

  • Formalin-fixed, paraffin-embedded rat kidney tissue stained for Transketolase using ab232819 at 10 µg/ml in immunohistochemical analysis.

    Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.

  • Formalin-fixed, paraffin-embedded rat colon tissue stained for Transketolase using ab232819 at 10 µg/ml in immunohistochemical analysis.

    Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.

  • Formalin-fixed, paraffin-embedded rat liver tissue stained for Transketolase using ab232819 at 10 µg/ml in immunohistochemical analysis.

    Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.

  • Formalin-fixed, paraffin-embedded rat spinal cord tissue stained for Transketolase using ab232819 at 10 µg/ml in immunohistochemical analysis.

    Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.

  • Anti-Transketolase antibody (ab232819) at 2 µg/ml + Recombinant rat Transketolase protein

    Predicted band size: 71 kDa

References

ab232819 has not yet been referenced specifically in any publications.

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