Anti-Transketolase antibody (ab233179)
Key features and details
- Rabbit polyclonal to Transketolase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Transketolase antibody
See all Transketolase primary antibodies -
Description
Rabbit polyclonal to Transketolase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Cynomolgus monkey, Orangutan -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Transketolase aa 1-300. Expressed in E.coli. N-terminal tags.
Database link: P29401 -
Positive control
- IHC-P: Human breast cancer, glioma, cerebrum, liver cancer, lung cancer, kidney and thyroid tissues. WB: A549 cell lysate; Recombinant human Transketolase protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab233179 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 68 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 68 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Sequence similarities
Belongs to the transketolase family. - Information by UniProt
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Database links
- Entrez Gene: 460443 Chimpanzee
- Entrez Gene: 445425 Cow
- Entrez Gene: 102136564 Cynomolgus monkey
- Entrez Gene: 7086 Human
- Entrez Gene: 21881 Mouse
- Entrez Gene: 100174016 Orangutan
- Entrez Gene: 64524 Rat
- Omim: 606781 Human
see all -
Alternative names
- EC 2.2.1.1 antibody
- FLJ34765 antibody
- TK antibody
see all
Images
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Anti-Transketolase antibody (ab233179) at 1 µg/ml + A549 (human lung carcinoma cell line) cell lysate
Secondary
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml
Predicted band size: 68 kDa -
Formalin-fixed, paraffin-embedded human thyroid tissue stained for Transketolase using ab233179 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Transketolase using ab233179 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human glioma tissue stained for Transketolase using ab233179 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human cerebrum tissue stained for Transketolase using ab233179 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Transketolase using ab233179 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Transketolase using ab233179 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for Transketolase using ab233179 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Anti-Transketolase antibody (ab233179) at 2 µg/ml + Recombinant human Transketolase protein
Predicted band size: 68 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233179 has not yet been referenced specifically in any publications.