Overview

  • Product name

    Anti-Translin/TSN antibody
    See all Translin/TSN primary antibodies
  • Description

    Rabbit polyclonal to Translin/TSN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Translin/TSN aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107811)

  • Positive control

    • This antibody gave a positive signal in the following human tissue lysates: Brain; Testis; Lymph Node as well as the following human whole cell lysates: Jurkat; HeLa; HepG2; MCF7.
  • General notes

     This product was previously labelled as Translin

     

Properties

Applications

Our Abpromise guarantee covers the use of ab96155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

Images

  • All lanes : Anti-Translin/TSN antibody (ab96155) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : Human brain tissue lysate - total protein (ab29466)
    Lane 6 : Human testis tissue lysate - total protein (ab30257)
    Lane 7 : Human lymph node tissue lysate - total protein (ab29871)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa
    Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • ICC/IF image of ab96155 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96155 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab96155 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for contacting Abcam.

I am sorry about the issues you were having with ab118917 in western blot. As we discussed I am sending a vial of ab96155 as a free of charge replacement. Your new order number is ***.

If there is anything else I can help you with, please let me know.

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