Anti-Transportin 1/MIP antibody (ab231669)
Key features and details
- Rabbit polyclonal to Transportin 1/MIP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Transportin 1/MIP antibody
See all Transportin 1/MIP primary antibodies -
Description
Rabbit polyclonal to Transportin 1/MIP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment (His-tag) corresponding to Cow Transportin 1/MIP aa 550-850. (Expressed in E.coli).
Database link: Q3SYU7 -
Positive control
- WB: HEK-293T and HeLa cell lysates; Recombinant cow Transportin 1/MIP protein. ICC/IF: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab231669 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 102 kDa.
|
|
ICC/IF |
Use a concentration of 5 - 20 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 102 kDa. |
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
Target
-
Function
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. -
Sequence similarities
Belongs to the importin beta family.
Contains 8 HEAT repeats.
Contains 1 importin N-terminal domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 767620 Cow
- Entrez Gene: 3842 Human
- Entrez Gene: 238799 Mouse
- Omim: 602901 Human
- SwissProt: Q3SYU7 Cow
- SwissProt: Q92973 Human
- SwissProt: Q8BFY9 Mouse
- Unigene: 482497 Human
see all -
Alternative names
- Importin 2 antibody
- Importin beta 2 antibody
- Importin beta-2 antibody
see all
Images
-
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells stained for Transportin 1/MIP using ab231669 at 20 µg/ml in ICC/IF.
-
Anti-Transportin 1/MIP antibody (ab231669) at 2 µg/ml + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 102 kDa -
Anti-Transportin 1/MIP antibody (ab231669) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 102 kDa -
Anti-Transportin 1/MIP antibody (ab231669) at 5 µg/ml + Recombinant cow Transportin 1/MIP protein
Predicted band size: 102 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab231669 has not yet been referenced specifically in any publications.