Overview

  • Product name

    Anti-TRAP alpha/TRAPA antibody [EPR5603]
    See all TRAP alpha/TRAPA primary antibodies
  • Description

    Rabbit monoclonal [EPR5603] to TRAP alpha/TRAPA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TRAP alpha/TRAPA aa 100-200.
    Database link: P43307

  • Positive control

    • A431, HL60, and T47D lysates; HepG2 cells
  • General notes

     

     

     This product was previously labelled as TRAP alpha

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5603
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.
    • Sequence similarities

      Belongs to the TRAP-alpha family.
    • Domain

      Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp781N23103 antibody
      • FLJ14232 antibody
      • FLJ22100 antibody
      • FLJ23034 antibody
      • FLJ78242 antibody
      • FLJ93042 antibody
      • PGP35 antibody
      • Signal sequence receptor alpha antibody
      • Signal sequence receptor alpha subunit antibody
      • Signal sequence receptor subunit alpha antibody
      • SSR 1 antibody
      • SSR alpha antibody
      • SSR-alpha antibody
      • SSR1 antibody
      • SSRA_HUMAN antibody
      • SSRalpha antibody
      • Translocon associated protein alpha antibody
      • Translocon associated protein alpha subunit antibody
      • Translocon associated protein subunit alpha antibody
      • Translocon-associated protein subunit alpha antibody
      • TRAP a antibody
      • TRAP-alpha antibody
      • TRAPa antibody
      • TRAPalpha antibody
      see all

    Images

    • All lanes : Anti-TRAP alpha/TRAPA antibody [EPR5603] (ab133238) at 1/10000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HL60 cell lysate
      Lane 3 : T47D cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 32 kDa
      Observed band size: 34 kDa
      why is the actual band size different from the predicted?

    • Flow cytometric analysis of TRAP alpha/TRAPA in permeabilized HepG2 cells, using ab133238 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Smith MD  et al. CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis. Dev Cell 44:217-232.e11 (2018). Read more (PubMed: 29290589) »
    • Ge YW  et al. Titanium particle-mediated osteoclastogenesis may be attenuated via bidirectional ephrin-B2/eph-B4 signaling in vitro. Int J Mol Med 42:2031-2041 (2018). Read more (PubMed: 30015911) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133238.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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