Recombinant Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-TRAP alpha/TRAPA antibody [EPR5604]
See all TRAP alpha/TRAPA primary antibodies -
Description
Rabbit monoclonal [EPR5604] to TRAP alpha/TRAPA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TRAP alpha/TRAPA aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P43307 -
Positive control
- HL60 and HepG2 cell lysates.
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General notes
This product was previously labelled as TRAP alpha
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5604 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa). |
Target
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Function
TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins. -
Sequence similarities
Belongs to the TRAP-alpha family. -
Domain
Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 6745 Human
- Entrez Gene: 107513 Mouse
- Entrez Gene: 361233 Rat
- Omim: 600868 Human
- SwissProt: P43307 Human
- SwissProt: Q9CY50 Mouse
- SwissProt: Q7TPJ0 Rat
- Unigene: 114033 Human
see all -
Alternative names
- DKFZp781N23103 antibody
- FLJ14232 antibody
- FLJ22100 antibody
see all
Images
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![Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)](https://www.abcam.com/ps/products/126/ab126775/Images/TRAP-alpha-Primary-antibodies-ab126775-1.jpg)
Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)All lanes : Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP
Predicted band size: 32 kDa -
![Other - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)](https://www.abcam.com/ps/products/126/ab126775/Images/TRAP-alpha-Primary-antibodies-ab126775-2.jpg)
Other - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhou L et al. MicroRNA-100-5p inhibits osteoclastogenesis and bone resorption by regulating fibroblast growth factor 21. Int J Mol Med 43:727-738 (2019). Read more (PubMed: 30535435) »
