Recombinant
RabMAb

Recombinant Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)

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Overview

  • Product name

    Anti-TRAP alpha/TRAPA antibody [EPR5604]
    See all TRAP alpha/TRAPA primary antibodies

  • Description

    Rabbit monoclonal [EPR5604] to TRAP alpha/TRAPA

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human TRAP alpha/TRAPA aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P43307

  • Positive control

    • HL60 and HepG2 cell lysates.

  • General notes

     

     

     This product was previously labelled as TRAP alpha

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 3.30 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR5604

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function

      TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.

    • Sequence similarities

      Belongs to the TRAP-alpha family.

    • Domain

      Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.

    • Cellular localization

      Endoplasmic reticulum membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • DKFZp781N23103 antibody
      • FLJ14232 antibody
      • FLJ22100 antibody
      • FLJ23034 antibody
      • FLJ78242 antibody
      • FLJ93042 antibody
      • PGP35 antibody
      • Signal sequence receptor alpha antibody
      • Signal sequence receptor alpha subunit antibody
      • Signal sequence receptor subunit alpha antibody
      • SSR 1 antibody
      • SSR alpha antibody
      • SSR-alpha antibody
      • SSR1 antibody
      • SSRA_HUMAN antibody
      • SSRalpha antibody
      • Translocon associated protein alpha antibody
      • Translocon associated protein alpha subunit antibody
      • Translocon associated protein subunit alpha antibody
      • Translocon-associated protein subunit alpha antibody
      • TRAP a antibody
      • TRAP-alpha antibody
      • TRAPa antibody
      • TRAPalpha antibody
      see all

    Images

    • Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)
      Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)
      All lanes : Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775) at 1/1000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP

      Predicted band size: 32 kDa

    • Other - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)
      Other - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Zhou L  et al. MicroRNA-100-5p inhibits osteoclastogenesis and bone resorption by regulating fibroblast growth factor 21. Int J Mol Med 43:727-738 (2019). Read more (PubMed: 30535435) »
    • Wang Y  et al. LncRNA LINC00311 Promotes the Proliferation and Differentiation of Osteoclasts in Osteoporotic Rats Through the Notch Signaling Pathway by Targeting DLL3. Cell Physiol Biochem 47:2291-2306 (2018). Read more (PubMed: 29975944) »
    See all 2 Publications for this product

    Publishing research using ab126775? Please let us know so that we can cite the reference in this datasheet.

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