Recombinant Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)
![Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)](https://www.abcam.com/ps/products/126/ab126775/Images/TRAP-alpha-Primary-antibodies-ab126775-1.jpg "Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5604] to TRAP alpha/TRAPA
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-TRAP alpha/TRAPA antibody [EPR5604]
See all TRAP alpha/TRAPA primary antibodies -
Description
Rabbit monoclonal [EPR5604] to TRAP alpha/TRAPA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide within Human TRAP alpha/TRAPA aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P43307 -
Positive control
- HL60 and HepG2 cell lysates.
-
General notes
This product was previously labelled as TRAP alpha
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5604 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa). |
Target
-
Function
TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins. -
Sequence similarities
Belongs to the TRAP-alpha family. -
Domain
Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6745 Human
- Entrez Gene: 107513 Mouse
- Entrez Gene: 361233 Rat
- Omim: 600868 Human
- SwissProt: P43307 Human
- SwissProt: Q9CY50 Mouse
- SwissProt: Q7TPJ0 Rat
- Unigene: 114033 Human
see all -
Alternative names
- DKFZp781N23103 antibody
- FLJ14232 antibody
- FLJ22100 antibody
see all
Images
-
Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)All lanes : Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa -
![OI-RD Scanning - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)](https://www.abcam.com/ps/products/126/ab126775/Images/TRAP-alpha-Primary-antibodies-ab126775-2.jpg)
OI-RD Scanning - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775) -
![Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)](https://www.abcam.com/ps/products/126/ab126775/Images/ab126775-3-benefits-of-recombinant-antibodies.png)
Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775)
Datasheets and documents
References (2)
ab126775 has been referenced in 2 publications.
- Zhou L et al. MicroRNA-100-5p inhibits osteoclastogenesis and bone resorption by regulating fibroblast growth factor 21. Int J Mol Med 43:727-738 (2019). PubMed: 30535435
- Wang Y et al. LncRNA LINC00311 Promotes the Proliferation and Differentiation of Osteoclasts in Osteoporotic Rats Through the Notch Signaling Pathway by Targeting DLL3. Cell Physiol Biochem 47:2291-2306 (2018). PubMed: 29975944
