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    trap1-antibody-ab151239.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-TRAP1 antibody (ab151239)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

Product price, shipping and contact information

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Western blot - Anti-TRAP1 antibody (ab151239)
  • Western blot - Anti-TRAP1 antibody (ab151239)
  • Western blot - Anti-TRAP1 antibody (ab151239)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRAP1 antibody (ab151239)

Key features and details

  • Rabbit polyclonal to TRAP1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Primary
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Anti-TRAP1 antibody [EPR5381] (ab109323)
Protein
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Recombinant human TRAP1 protein (ab123775)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TRAP1 antibody
    See all TRAP1 primary antibodies
  • Description

    Rabbit polyclonal to TRAP1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 392-704 of Human TRAP1 (Uniprot: Q12931).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji and Mouse brain whole cell lysates; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • A-431 whole cell lysate (ab30132)
    • Mouse brain tissue lysate - total protein (ab30151)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant human TRAP1 protein (ab123775)
  • Related Products

    • Recombinant human TRAP1 protein (ab123775)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Mouse
Human
All applications
Rat
Chicken
Cow
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Chaperone that expresses an ATPase activity.
  • Tissue specificity

    Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
  • Sequence similarities

    Belongs to the heat shock protein 90 family.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession Q12931 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 416666 Chicken
    • Entrez Gene: 514472 Cow
    • Entrez Gene: 10131 Human
    • Entrez Gene: 68015 Mouse
    • Entrez Gene: 287069 Rat
    • Omim: 606219 Human
    • SwissProt: Q2TBI4 Cow
    • SwissProt: Q12931 Human
    • SwissProt: Q9CQN1 Mouse
    • SwissProt: Q5XHZ0 Rat
    • Unigene: 30345 Human
    • Unigene: 123366 Mouse
    • Unigene: 144695 Rat
    see all
  • Alternative names

    • Heat shock protein 75 kDa antibody
    • Heat shock protein 75 kDa, mitochondrial antibody
    • HSP 75 antibody
    • HSP75 antibody
    • HSP90L antibody
    • mitochondrial antibody
    • TNF receptor associated protein 1 antibody
    • TNFR-associated protein 1 antibody
    • TRAP-1 antibody
    • Trap1 antibody
    • TRAP1_HUMAN antibody
    • Tumor necrosis factor type 1 receptor-associated protein antibody
    see all

Images

  • Western blot - Anti-TRAP1 antibody (ab151239)
    Western blot - Anti-TRAP1 antibody (ab151239)
    All lanes : Anti-TRAP1 antibody (ab151239) at 1/500 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : TRAP1 Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 80 kDa

  • Western blot - Anti-TRAP1 antibody (ab151239)
    Western blot - Anti-TRAP1 antibody (ab151239)
    All lanes : Anti-TRAP1 antibody (ab151239) at 1/1500 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Western blot - Anti-TRAP1 antibody (ab151239)
    Western blot - Anti-TRAP1 antibody (ab151239)
    Anti-TRAP1 antibody (ab151239) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-TRAP1 antibody (ab151239)
    Immunocytochemistry/ Immunofluorescence - Anti-TRAP1 antibody (ab151239)
    Immunofluorescence analysis of methanol-fixed HeLa cells labeling TRAP1 with ab151239 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (3)

    Publishing research using ab151239? Please let us know so that we can cite the reference in this datasheet.

    ab151239 has been referenced in 3 publications.

    • Li X  et al. Epigenetic regulation of NfatC1 transcription and osteoclastogenesis by nicotinamide phosphoribosyl transferase in the pathogenesis of arthritis. Cell Death Discov 5:62 (2019). PubMed: 30774990
    • Lin MH  et al. Immunological evaluation of a novel HLA-A2 restricted phosphopeptide of tumor associated Antigen, TRAP1, on cancer therapy. Vaccine X 1:100017 (2019). PubMed: 31384738
    • Wang Y  et al. Overexpression of mitochondrial Hsp75 protects neural stem cells against microglia-derived soluble factor-induced neurotoxicity by regulating mitochondrial permeability transition pore opening in vitro. Int J Mol Med 36:1487-96 (2015). WB . PubMed: 26500047

    Customer reviews and Q&As

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