Anti-TRAP1 antibody (ab151239)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-TRAP1 antibody
See all TRAP1 primary antibodies -
DescriptionRabbit polyclonal to TRAP1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 392-704 of Human TRAP1 (Uniprot: Q12931).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji and Mouse brain whole cell lysates; HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab151239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 80 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionChaperone that expresses an ATPase activity.
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Tissue specificityFound in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
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Sequence similaritiesBelongs to the heat shock protein 90 family.
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Cellular localizationMitochondrion.
- Information by UniProt
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Database links
- Entrez Gene: 416666 Chicken
- Entrez Gene: 514472 Cow
- Entrez Gene: 10131 Human
- Entrez Gene: 68015 Mouse
- Entrez Gene: 287069 Rat
- Omim: 606219 Human
- SwissProt: Q2TBI4 Cow
- SwissProt: Q12931 Human
see all -
Alternative names
- Heat shock protein 75 kDa antibody
- Heat shock protein 75 kDa, mitochondrial antibody
- HSP 75 antibody
see all
Images
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Western blot - Anti-TRAP1 antibody (ab151239)All lanes : Anti-TRAP1 antibody (ab151239) at 1/500 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : TRAP1 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 80 kDa -
Western blot - Anti-TRAP1 antibody (ab151239)All lanes : Anti-TRAP1 antibody (ab151239) at 1/1500 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 80 kDa
7.5% SDS PAGE -
Western blot - Anti-TRAP1 antibody (ab151239)Anti-TRAP1 antibody (ab151239) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-TRAP1 antibody (ab151239)Immunofluorescence analysis of methanol-fixed HeLa cells labeling TRAP1 with ab151239 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
References
This product has been referenced in:
- Li X et al. Epigenetic regulation of NfatC1 transcription and osteoclastogenesis by nicotinamide phosphoribosyl transferase in the pathogenesis of arthritis. Cell Death Discov 5:62 (2019). Read more (PubMed: 30774990) »
- Wang Y et al. Overexpression of mitochondrial Hsp75 protects neural stem cells against microglia-derived soluble factor-induced neurotoxicity by regulating mitochondrial permeability transition pore opening in vitro. Int J Mol Med 36:1487-96 (2015). WB . Read more (PubMed: 26500047) »
