Anti-TRAP1 antibody (ab151239)
Key features and details
- Rabbit polyclonal to TRAP1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TRAP1 antibody
See all TRAP1 primary antibodies -
Description
Rabbit polyclonal to TRAP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB MouseHuman
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 392-704 of Human TRAP1 (Uniprot: Q12931).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji and Mouse brain whole cell lysates; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Mouse
Human
|
| All applications |
Rat
Chicken
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa. |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
Chaperone that expresses an ATPase activity. -
Tissue specificity
Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 416666 Chicken
- Entrez Gene: 514472 Cow
- Entrez Gene: 10131 Human
- Entrez Gene: 68015 Mouse
- Entrez Gene: 287069 Rat
- Omim: 606219 Human
- SwissProt: Q2TBI4 Cow
- SwissProt: Q12931 Human
see all -
Alternative names
- Heat shock protein 75 kDa antibody
- Heat shock protein 75 kDa, mitochondrial antibody
- HSP 75 antibody
see all
Images
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Western blot - Anti-TRAP1 antibody (ab151239)All lanes : Anti-TRAP1 antibody (ab151239) at 1/500 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : TRAP1 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 80 kDa -
Western blot - Anti-TRAP1 antibody (ab151239)All lanes : Anti-TRAP1 antibody (ab151239) at 1/1500 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 80 kDa
7.5% SDS PAGE -
Western blot - Anti-TRAP1 antibody (ab151239)Anti-TRAP1 antibody (ab151239) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-TRAP1 antibody (ab151239)Immunofluorescence analysis of methanol-fixed HeLa cells labeling TRAP1 with ab151239 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Protocols
References (3)
ab151239 has been referenced in 3 publications.
- Li X et al. Epigenetic regulation of NfatC1 transcription and osteoclastogenesis by nicotinamide phosphoribosyl transferase in the pathogenesis of arthritis. Cell Death Discov 5:62 (2019). PubMed: 30774990
- Lin MH et al. Immunological evaluation of a novel HLA-A2 restricted phosphopeptide of tumor associated Antigen, TRAP1, on cancer therapy. Vaccine X 1:100017 (2019). PubMed: 31384738
- Wang Y et al. Overexpression of mitochondrial Hsp75 protects neural stem cells against microglia-derived soluble factor-induced neurotoxicity by regulating mitochondrial permeability transition pore opening in vitro. Int J Mol Med 36:1487-96 (2015). WB . PubMed: 26500047
